Slit2 항체 (F-7) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 Slit2을 검출할것을 권장합니다.

Slit2 항체 (F-7): sc-514499

Slit2 항체 (F-7) is rated 1.0 out of 5 by 2.
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데이터 시트

    • 안티-Slit2 항체 F-7 는 마우스 monoclonal IgM κ Slit2 항체, 2간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Slit2에서 C-terminus에 위치한 1441-1464의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
    • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 Slit2을 검출할것을 권장합니다.
    • Slit2 (F-7): sc-514499무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
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Does the Slit2 Antibody Slit2 Antikörper (F-7): sc-514499 work in IHC with FFPE tissue? Or is there any other SantaCruz antibody for this purpose?

Asked by: Helmut Klocker
Thank you for your question. We recommend using this mouse monoclonal antibody, Slit2 (F-7): sc-514499, for IF, or immunofluorescence staining on fresh frozen tissues or cells fixed in acetone or methanol. The antibody is not recommended for IHC(P) on formalin-fixed paraffin-embedded tissue sections as it has not been tested for that application. It may work, but it has not been tested. We recommend using a starting dilution of 1:50 (range of 1:50-1:500) for IF or IHC. If you would like to try a sample of this product, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-10-18

What application is the blocking peptide sc-514499 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from 需要Slit2 抗体 (F-7): sc-514499小样 需要Slit2 抗体 (F-7): sc-514499小样。15304031461.................................................
Date published: 2017-12-13
Rated 1 out of 5 by from This Slit2 antibody is not great for IF or WB I was sent this antibody for trying in IF and WB. We have tested and found that it did not pick up Slit2 in the mouse kidney tissue at the dilution of 1:100. Western blot assay did not find bends of human Slit2 stably expressed in HEK293 cells.
Date published: 2017-07-20
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