Skeletal Muscle Myosin 항체 (F59) WB, IP, IF와 IHC(P)으로 mouse, rat, human와 avian origin의 fast fiber–specific skeletal muscle myosin heavy chain을 검출할것을 권장합니다.

Skeletal Muscle Myosin 항체 (F59): sc-32732

Skeletal Muscle Myosin 항체 (F59) is rated 4.8 out of 5 by 10.
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데이터 시트

    • 안티-Skeletal Muscle Myosin 항체 F59 는 마우스 monoclonal IgG1 κ Skeletal Muscle Myosin 항체, 47간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against Myosin purified from pectoralis muscle of adult white Leghorn chicken origin
    • 안티-Skeletal Muscle Myosin 항체 (F59)는 WB, IP, IF and IHC(P)으로 mouse, rat, human and avian유래의 fast fiber–specific skeletal muscle myosin heavy chain 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Skeletal Muscle Myosin 항체 (F59)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Skeletal Muscle Myosin (F59): sc-32732무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Skeletal Muscle Myosin Antibody (F59) for WB and IHC(P) applications. These reagents are now offered in bundles with Skeletal Muscle Myosin Antibody (F59) (see ordering information below).
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Does this antibody detect any specific myosin heavy chain? Such as MYH1, MYH6, MYH7, MYH15, MYH2, MYH3, MYH4 etc?

Asked by: Eric Spinetti
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. This clone is recommended for the detection of fast fiber–specific skeletal muscle myosin heavy chain. The exact myosin heavy chain genes/proteins that are targeted are not known.
Answered by: Technical Support
Date published: 2019-09-20

I am interested in performing a immunoprecipitation of MYH6 using this antibody. However, I don't see any validation of this technique in your website or in any cited papers. Do you have any data demonstrating the efficiency of F59 (sc-32732) for IP?

Asked by: Anonymous
This primary antibody is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-09-20

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 4 out of 5 by from Good signal This antibody works well on human and mouse samples in WB
Date published: 2018-08-01
Rated 4 out of 5 by from Great features! The antibody is work well in WB at 1:100 of C2C12 cells.
Date published: 2017-08-31
Rated 5 out of 5 by from good WB dilution 1/1000, mouse heart tissue detected clean band
Date published: 2017-05-09
Rated 5 out of 5 by from Sehr gut Application in mouse cells, very clear immunolabelling.
Date published: 2017-04-05
Rated 5 out of 5 by from Outstanding Strong western blot data, low background; nice IHC staining
Date published: 2017-01-29
Rated 5 out of 5 by from Positive result in WB I bought it for years,tested it in WB with pig dorsal muscles,it is very wonderful
Date published: 2016-12-13
Rated 5 out of 5 by from Western blot and IF publications using mouse Western blot and IF publications using mouse C2C12 myoblasts. -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Publishable IHC data in mouse cardiomyocytes Publishable IHC data in mouse cardiomyocytes. -SCBT Publication Review
Date published: 2015-02-08
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