Ribosomal Protein S18 CRISPR/Cas9 KO Plasmid (h): sc-406494
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein S18 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ribosomal Protein S18 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein S18 CRISPR/Cas9 KO Plasmid (h) | sc-406494 | 20 µg | $397.00 |
Overview
RPS18 encodes ribosomal protein S18, an essential component of the 40S small ribosomal subunit that helps stabilize rRNA structure and supports accurate decoding during translation initiation and elongation. As part of ribosome biogenesis and global protein synthesis, RPS18 contributes to proteostasis, cell growth, and stress-adaptive translational control pathways. Altered expression or perturbation of ribosomal proteins can influence nucleolar stress signaling and p53-dependent checkpoints, linking ribosome dysfunction to changes in proliferation and apoptosis programs. Dysregulated ribosome biogenesis and translation are recurrent features of cancer and ribosomopathies, making RPS18 a useful node for studying how core translational machinery interfaces with disease-associated cellular states.
Ribosomal Protein S18 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RPS18 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RPS18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RPS18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ribosomal Protein S18 protein expression.
This CRISPR knockout system enables efficient generation of RPS18-deficient cell models for investigation of Ribosomal Protein S18 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RPS18 exon(s) critical for Ribosomal Protein S18 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RPS18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ribosomal Protein S18 CRISPR/Cas9 KO Plasmid (h) and Ribosomal Protein S18 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RPS18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ribosomal Protein S18 HDR Plasmid (h) and Ribosomal Protein S18 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RPS18 homology arms to support homology-directed repair at defined RPS18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.