POLR2E 항체 (B-5) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat, human 와 avian origin의 POLR2E을 검출할것을 권장합니다.

POLR2E 항체 (B-5): sc-390979

POLR2E 항체 (B-5) is rated 4.0 out of 5 by 2.
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데이터 시트

    • 안티-POLR2E 항체 B-5 는 마우스 monoclonal IgG2a (kappa light chain) POLR2E 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 POLR2E의 7-210 아미노산을 항원으로 사용하였습니다.
    • POLR2E 항체 (B-5)는 WB, IP, IF, IHC(P) and ELISA으로mouse, rat, human and avian유래의 POLR2E 를 감지하는 데에 추천한다 .; 이외에, equine, bovine, porcine and avian등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-POLR2E 항체 (B-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • POLR2E (B-5): sc-390979무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for POLR2E Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with POLR2E Antibody (B-5) (see ordering information below).
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Can POLR2E (B-5): sc-390979 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, POLR2E (B-5): sc-390979 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from good features I applied for the samples several days ago and they gave it to me immediately. very good and sensitive.
Date published: 2018-01-31
Rated 5 out of 5 by from Produced positive Western blot data of POLR2E Produced positive Western blot data of POLR2E expression in MCF7 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2014-04-21
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