POLR2B 항체 (E-12) recommended for detection of by WB, IP, IF와 ELISA으로 mouse, rat, human와 origin의 POLR2B을 검출할것을 권장합니다.

POLR2B 항체 (E-12): sc-166803

POLR2B 항체 (E-12) is rated 5.0 out of 5 by 2.
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데이터 시트

    • 안티-POLR2B 항체 E-12 는 마우스 monoclonal IgG2a κ POLR2B 항체, 4간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 POLR2B에서 N-terminus에 위치한 184-384 아미노산을 항원으로 사용하였습니다.
    • POLR2B 항체 (E-12)는 WB, IP, IF and ELISA으로mouse, rat, human and 유래의 POLR2B 를 감지하는 데에 추천한다 ; 이외에, equine, canine, bovine, porcine and avian등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-POLR2B 항체 (E-12)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-166803 X, 200 µg/0.1 ml)이 있습니다.
    • POLR2B (E-12): sc-166803무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for POLR2B Antibody (E-12) for WB applications. These reagents are now offered in bundles with POLR2B Antibody (E-12) (see ordering information below).
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When should the TransCruz reagent sc-166803 X be used instead of the standard form sc-166803?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from great RPB2 antibody I tried this 1:5000 with Hela NE western, works great
Date published: 2016-11-30
Rated 5 out of 5 by from Satisfactory Western blot data of POLR2B Satisfactory Western blot data of POLR2B expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-03-01
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