POLR1E 항체 (C-11) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 POLR1E을 검출할것을 권장합니다.

POLR1E 항체 (C-11): sc-398270

POLR1E 항체 (C-11) is rated 5.0 out of 5 by 1.
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데이터 시트

    • 안티-POLR1E 항체 C-11 는 마우스 monoclonal IgG2a κ POLR1E 항체, 2간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 POLR1E에서 N-terminus에 위치한 8-113 아미노산을 항원으로 사용하였습니다.
    • 안티-POLR1E 항체 (C-11)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 POLR1E 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-POLR1E 항체 (C-11)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-398270 X, 200 µg/0.1 ml)이 있습니다.
    • POLR1E (C-11): sc-398270무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for POLR1E Antibody (C-11) for WB applications. These reagents are now offered in bundles with POLR1E Antibody (C-11) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-398270: POLR1E (C-11) monoclonal antibody?

Asked by: Cweed
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Very good Western blot data of POLR1E expression Very good Western blot data of POLR1E expression in JAR, HeLa, K-562 and Jurkat whole cell lysates and HeLa nuclear extract . -SCBT QC
Date published: 2013-03-28
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