PINK1 Double Nickase Plasmid (h): sc-400739-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PINK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PINK1 Double Nickase Plasmid (h) and PINK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PINK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PINK1 Antibody (38CT20.8.5): sc-517353
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PINK1 Double Nickase Plasmid (h) | sc-400739-NIC | 20 µg | $410.00 | |||
PINK1 Double Nickase Plasmid (h2) | sc-400739-NIC-2 | 20 µg | $410.00 |
PINK1 (PTEN-induced kinase 1) encodes a mitochondria-targeted serine/threonine kinase that functions as a key sensor of mitochondrial damage and a regulator of organelle quality control. Upon loss of mitochondrial membrane potential, PINK1 accumulates on the outer mitochondrial membrane and promotes PARKIN-dependent ubiquitination of mitochondrial substrates, initiating mitophagy and remodeling of mitochondrial dynamics. Through this pathway, PINK1 influences oxidative stress responses, bioenergetic homeostasis, and inflammatory signaling linked to mitochondrial dysfunction. Disruption of PINK1 activity is strongly associated with early-onset Parkinson’s disease and is widely studied in models of neurodegeneration and mitochondrial pathology.
PINK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PINK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PINK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PINK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PINK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.