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Phosphotyrosine Antibody (PY99) | SCBT - Santa Cruz Biotechnology
Phosphotyrosine 항체 (PY99) WB, IP, IF와 IHC(P)으로 phosphotyrosine-containing proteins 을 검출할것을 권장합니다.

Phosphotyrosine 항체 (PY99): sc-7020

p-Tyr 항체 (PY99) is rated 4.7 out of 5 by 31.
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데이터 시트

    • Phosphotyrosine 항체(PY99) 는 마우스 monoclonal IgG2bκ, 2,686간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against p-Tyr
    • Phosphotyrosine 항체(PY99)는 WB, IP, IF and IHC(P)으로 phosphotyrosine-containing proteins 를 감지하는 데에 추천한다 .; phosphoserine or phosphothreonine와 교차반응이 없습니다.
    • Anti-Phosphotyrosine Antibody (PY99) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • WB, IHC(P)와 ELISA에 사용가능한 biotin결합제품 IF, IHC(P) 와 FCM에 사용가능한 Alexa Fluor® 405결합제품 이 있습니다.
    • IF, IHC(P) 와 FCM에 사용가능한 Alexa Fluor® 405 결합제품이 있습니다.
    • Phosphotyrosine (PY99): sc-7020무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Phosphotyrosine Antibody (PY99). This work is in progress.
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What dilutions do you recommend for both the primary and secondary antibodies in .5% BSA for Western Blotting.

Asked by: carolineh325
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Anonymous
Date published: 2022-12-19

Is there anybody know the difference between PY20 and PY99?

Asked by: shaw
Thank you for your question. p-Tyr Antibody (PY99) is recommended for detection of phosphotyrosine-containing proteins by WB, IP, IF and IHC(P); non cross-reactive with phosphoserine or phosphothreonine. p-Tyr Antibody (PY20) is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM.
Answered by: Technical Support 12
Date published: 2022-06-17

Dear Santa Cruze Do i have to boil/unboil my cell lysate before i use this p-Tyr(PY99) for western blot application?

Asked by: Kenny C
Thank you for your question. Yes, you should boil your lysate before loading it onto your gel.
Answered by: Tech Service
Date published: 2019-11-25

When I use this antibody for immunohistochemistry. Is antigen retrieval necessary?

Asked by: AH2019
Thank you for your question. Yes, we recommend performing an antigen retrieval step if you are doing IHC on formalin fixed paraffin embedded tissue sections.
Answered by: Tech Service
Date published: 2019-10-15

Can I use 10% milk to block when using this antibody? I am interested in getting it conjugated with the alexa 790 to be used with LI-COR®Odyssey®.

Asked by: nsal
Thank you for your question. We recommend blocking in 5% milk with TBS-T, and it should be fine with the LiCOR Odyssey.
Answered by: Tech Service
Date published: 2019-09-24

Is there any species specificity for this antibody? Or is there any cross-reactivity?

Asked by: beni
Thank you for your question. This antibody has broad species reactivity and is specific for phosphotyrosine-containing proteins; non cross-reactive with phosphoserine or phosphothreonine.
Answered by: Technical Support
Date published: 2019-09-11

I want to measure insulin sensitivity and several tyrosine domains are phosphorylated in response to insulin. For example in both insulin receptors and in IRS. Does the antibody detetct all p-tyr, and I differentiate between them depending on weight?

Asked by: Ahmed_hassan
Thank you for your question. Yes, you are correct, this mouse monoclonal antibody, p-Tyr (PY99): sc-7020, specifically detects phosphotyrosine-containing proteins (and does not cross-reactive with phosphoserine or phosphothreonine-containing proteins). If you are using the antibody for Western blotting, then the molecular weight you detect in your samples is representative of different phosphorylated proteins. You would need to do further testing to identify which specific proteins are phosphorylated in your samples.
Answered by: Technical Support
Date published: 2021-04-06

What secondary reagent should I use with p-Tyr Antibody (PY99) for Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-15
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Rated 3 out of 5 by from Pretty good antibody The antibody works pretty well upon IP whereas on total cellular extract is too weak.
Date published: 2020-10-07
Rated 5 out of 5 by from detection of phosphotyrosine-containing proteins I recommend for detection of phosphotyrosine-containing proteins!
Date published: 2019-12-04
Rated 4 out of 5 by from good pTr antibody Works well to detect tyrosine phosphorylation in whole cell or tissue lysates.
Date published: 2018-05-17
Rated 5 out of 5 by from good for WB I use 1:1000 in 5%BSA, it is less specific for mouse lung tissue, I haven't try primary cell but I hope it works.
Date published: 2018-03-12
Rated 4 out of 5 by from Good for WB I tried a 1/500 dilution in 3% BSA and it worked well. Good signal.
Date published: 2017-10-24
Rated 5 out of 5 by from IHC detection IHC detected the expression of P-Tyr in gastric carcinoma tissues ,it gave cytoplasmic staining ,good signal for us.
Date published: 2017-09-16
Rated 5 out of 5 by from staining IHC(1/100) staining in human skin tissu, very sensitive
Date published: 2017-09-08
Rated 5 out of 5 by from very good Gives clear for nuclear extracts in western blot; had a strong signal in the western blot.
Date published: 2017-08-17
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