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Phosphotyrosine Antibody (PY20) | SCBT - Santa Cruz Biotechnology
p-Tyr 항체 (PY20) WB, IP, IF, IHC(P) 와FCM으로broad species을 검출할것을 권장합니다.

p-Tyr 항체 (PY20): sc-508

p-Tyr 항체 (PY20) is rated 4.8 out of 5 by 19.
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데이터 시트

    • p-Tyr 항체(PY20) 는 마우스 monoclonal IgG2bκ, 1,332간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against p-Tyr
    • p-Tyr 항체 (PY20)는 WB, IP, IF, IHC(P) and FCM으로 broad species의 phosphotyrosine-containing proteins 를 감지하는 데에 추천한다 .
    • Anti-p-Tyr Antibody (PY20) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • IF, IHC(P) 와 FCM에 사용가능한 Alexa Fluor® 405 결합제품이 있습니다.
    • p-Tyr (PY20): sc-508무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Tyr Antibody (PY20) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Tyr Antibody (PY20) (see ordering information below).
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Hello! Please tell me if p-Tyr Antibody (PY20): sc-508 is working in ELISA assay. If yes, could you recommend me a starting dilution range! I want to know if the p-Tyr Antibody (PY20) HRP variant is recommended for ELISA and in what dilution.

Asked by: Rodica
Thank you for your question. p-Tyr Antibody (PY20): sc-508 is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM. It is also available conjugated to HRP for ELISA. The starting dilution is 1:30, with a dilution range of 1:30-1:3000.
Answered by: Tech Support Europe
Date published: 2020-11-27

Which phosphotyrosine antibody is more sensitive in Western Blot: p-Tyr (PY99) or p-Tyr (PY20)? Which concentration is the recommended?

Asked by: AngelesMZ
Thank you for your question. Both p-Tyr Antibody (PY20): sc-508 and p-Tyr Antibody (PY99): sc-7020 are featured in several hundred publications and are recommended for western blot and other applications. Both antibodies have a recommended starting dilution of 1:200 and work in a dilution range 1:100-1:1000. Either antibody is perfectly suited for western blotting, so you could order one or the other or get in touch with our technical service to arrange for free samples of the two antibodies.
Answered by: Technical Support Europe
Date published: 2019-04-12

for blocking, what would you recommend? Many people recommend 5% of BSA for phospho-protein..( should I stick with this rule.). but some phospho-protein can worked well with.. Skim milk (depends the antibody protocol..)

Asked by: blessing
Thank you for your question. We recommend using 1% milk, 1% BSA in 0.05% Tween-20 for blocking and antibody incubations.
Answered by: Technical Support
Date published: 2019-02-01

EGF-treated とは、具体的にどのようにするものでしょうか。ご教授お願い致します。

Asked by: k 2018
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Can I use p-Tyr Antibody (PY20): sc-508 for immunohistochemical staining of mouse tissue

Asked by: Anonymous
We recommend using a directly conjugated mouse monoclonal primary antibody. See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/p-tyr-antibody-py99
Answered by: Tech Service 6
Date published: 2016-12-16

What is the recommended secondary detection reagent in Western blot?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-16
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Rated 5 out of 5 by from Always reliable! I have relied on you for over 20 years and you have always delivered an excellent product on time. Your phone staff is friendly and helpful.
Date published: 2018-03-08
Rated 4 out of 5 by from Very good I have used Py20 Antibody from Santa and it worked great.
Date published: 2017-12-14
Rated 4 out of 5 by from As expected Gives WB bands as expected at 1:500 dilution in 5% SA (block in non-fat dry milk).
Date published: 2017-12-02
Rated 5 out of 5 by from Very Strong Signal I compare different products for art antibody found p-Tyr antibody is the most sensitive, sc-508 is the most reliable quality.
Date published: 2017-10-28
Rated 5 out of 5 by from Excellent antibody Strong signal and low background on immunoprecipitated protein.
Date published: 2017-09-01
Rated 5 out of 5 by from good result antibody It is an excellent antibody. gives a single band in my hand.
Date published: 2017-05-27
Rated 5 out of 5 by from antibody for WB I use the dilution 1:800, get very clean bands.I like
Date published: 2017-05-07
Rated 5 out of 5 by from High Quality Great antibody for detection of any protein with a phosphorylated Tyrosine, can be used in a number of applications, not species specific.
Date published: 2016-12-20
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