p-EGFR 항체 (9H2) WB, IP와 IF으로 mouse, rat, human와 canine origin의 Tyr 1173 phosphorylated EGFR을 검출할것을 권장합니다.

p-EGFR 항체 (9H2): sc-57545

p-EGFR 항체 (9H2) is rated 4.7 out of 5 by 7.
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데이터 시트

    • p-EGFR 항체 9H2 는 마우스 monoclonal IgG1, 8간행물에 인용, 이며 50 µg/0.5 ml으로 제공합니다.
    • raised against an EFGR phosphopeptide of human origin
    • WB, IP와 IF으로 mouse, rat, human와 canine origin의 Tyr 1173 phosphorylated EGFR을 검출할것을 권장합니다.; non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins와 교차반응이 없습니다.
    • See EGFR (A-10): sc-373746 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • p-EGFR (9H2): sc-57545무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for p-EGFR Antibody (9H2) for WB applications. These reagents are now offered in bundles with p-EGFR Antibody (9H2) (see ordering information below).

How much protein do you recommend loading on the gel for Western blot detection using p-EGFR (9H2): sc-57545 monoclonal antibody?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-02-02
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Rated 4 out of 5 by from Good The antibody was used for Western Blot in breast carcinoma cells, it works at a concentration of 1: 100.
Date published: 2020-10-16
Rated 4 out of 5 by from Works well used for WB looking at mouse tissue samples using 1:200. Provides a faint but clear band.
Date published: 2018-03-22
Rated 5 out of 5 by from Great antibody i got this antibody as free trial size and worked so well on mouse mammary tissue lysates.
Date published: 2017-12-15
Rated 5 out of 5 by from Great phospho-detection Great, published antibody for p-EGFR detection recommended for use in mouse, rat, human, and canine. Strong bands in WB for phosphorylated samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Working great with dSTORM IF with HeLa and Working great with dSTORM IF with HeLa and Cos7 cells.
Date published: 2015-03-30
Rated 5 out of 5 by from Good Western blot analysis of EGFR phosphorylation Good Western blot analysis of EGFR phosphorylation in non-stimulated, EGF stimulated and pervanadate stimulated A549 whole cell lysates. - SCBT QC
Date published: 2014-02-09
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