Oct-6 항체 (B-7) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Oct-6을 검출할것을 권장합니다.

Oct-6 항체 (B-7): sc-376143

Oct-6 항체 (B-7) is rated 4.0 out of 5 by 4.
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데이터 시트

    • 안티-Oct-6 항체 B-7 는 마우스 monoclonal IgG2a κ Oct-6 항체, 2간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Oct-6에서 에 가까운 111-160 아미노산을 항원으로 사용하였습니다.
    • 안티-Oct-6 항체 (B-7)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Oct-6 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Oct-6 항체 (B-7)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-376143 X, 200 µg/0.1 ml)이 있습니다.
    • Oct-6 (B-7): sc-376143무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Oct-6 Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Oct-6 Antibody (B-7) (see ordering information below).
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Can Oct-6 (B-7): sc-376143 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, Oct-6 (B-7): sc-376143 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 1 out of 5 by from No signal (Immunofluorescence on cryosections) It gave no specific signal on mouse embryonic spinal cord cryosections, without significant background, at dilutions 1/100 to 1/1000 (with or without antigen retrieval procedure). Nkx6.2 is present in motor neurons and in ventral interneurons in the developing spinal cord.
Date published: 2017-08-10
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HepG2. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Great antibody for paraffin embedded sections Great antibody for paraffin embedded sections. Antibody gives a strong nuclear and cytoplasmic signal in human skin tissue. -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from Good signal strength by Western in Jurkat and HL Good signal strength by Western in Jurkat and HL-60 whole cell lysates. -SCBT QC
Date published: 2014-05-21
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