NP-CGG, Ratio 10-19, is a 4-Hydroxy-3-nitrophenylacetyl hapten conjugated to CGG via amide bonds to lysine.
Absorption Maximum (Lambda Max): 430 nm Extinction Coefficient at Lambda max: 4230/M/cm
1. Vora, K A., et al., 1997. Amplified follicular immune complex deposition in mice lacking the Fc receptor gamma-chain does not alter maturation of the B cell response. Journal of immunology (Baltimore, Md. : 1950). 159(5): 2116-24. PMID: 9278297 2. Rada, C., et al., 1998. Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting. Immunity. 9(1): 135-41. PMID: 9697843
Conjugation is determined at pH 8.5 by measuring NP absorbance at 430nm, using the extinction coefficient of NP= 4.23 x 10E+03. The conjugation ratio is given as a molar ratio of NP to CGG assuming the molecular weight of CGG is 150,000.
Store solution in aliquots at -20°C. Protect from light.
This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant.
Soluble in PBS buffer (1 mg/ml), water (1 mg/ml), and other neutral buffers (1 mg/ml).