MYH3 항체 (F1.652) WB, IP, IF와 IHC(P)으로 mouse, rat, human와 rabbit origin의 myosin heavy chain encoded by MYH3을 검출할것을 권장합니다.

MYH3 항체 (F1.652): sc-53091

MYH3 항체 (F1.652) is rated 5.0 out of 5 by 6.
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데이터 시트

    • MYH3 항체(F1.652) 는 마우스 monoclonal IgG1κ, 33간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against fetal skeletal muscle myosin of human origin
    • 안티-MYH3 항체 (F1.652)는 WB, IP, IF and IHC(P)으로 mouse, rat, human and rabbit유래의 myosin heavy chain encoded by MYH3 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-MYH3 항체 (F1.652)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • MYH3 (F1.652): sc-53091무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MYH3 Antibody (F1.652) for WB and IHC(P) applications. These reagents are now offered in bundles with MYH3 Antibody (F1.652) (see ordering information below).
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Is the antigen for this antibody for the entire 1940 AA sequence? I need to match the sequence used to equine sequence to see if I can use it. thank you,

Asked by: Kgardner
Thank you for your question. This mouse monoclonal antibody is not raised against an amino acid sequence, but rather is raised against a preparation of fetal skeletal muscle myosin of human origin. The exact epitope recognized by this monoclonal antibody has not been determined. Most product citations use this antibody in mouse and rat samples, and we are not aware of any product citations in equine samples at this time. Reactivity with equine would have to be determined experimentally.
Answered by: Technical Support
Date published: 2020-02-12

Do you use Heat induce epitiope retrieval for MYH3 (F1.652):sc-53091.

Asked by: MIGUEL VARGAS
Thank you for your question. Yes, we recommend antigen retrieval using sodium citrate and heat for sc-53091.
Answered by: Technical Support
Date published: 2018-12-14

Can this antibody be used in IHC with paraffin-embedded tissue sections?

Asked by: AbPolly
Thank you for your inquiry. This antibody has not been validated in house for use in IHC(P). For any publications in this application and further details, please contact our Technical Service Department.
Answered by: Technical Support 13
Date published: 2018-07-23
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Rated 5 out of 5 by from Sensitive antibody This MYH3 antibody is easy to use without any need to modify the protocol. It is specific and sensitive to the target protein at 200kda by 1:500.
Date published: 2017-07-29
Rated 5 out of 5 by from clear band Strong and clear band has been gotten by this specific antibody for WB anyasis.
Date published: 2017-05-28
Rated 5 out of 5 by from Strong western data Nice product for western blot applications, low background
Date published: 2017-01-03
Rated 5 out of 5 by from A perfect choice for your Western blot! This antibody is your best choice for detecing the myosin heavy chain. It is reactive in several different species, but provides clear specific bands with each sample. It has been published with several times!
Date published: 2016-12-28
Rated 5 out of 5 by from signal via Western blot analysis of MHC expression signal via Western blot analysis of MHC expression in L8 whole cell lysate. -SCBT QC
Date published: 2015-01-06
Rated 5 out of 5 by from ICC publication using mouse satellite cell ICC publication using mouse satellite cell from hind limb skeletal muscles -SCBT Publication Review
Date published: 2013-06-05
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