MYH15 항체 (C-8) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 MYH15을 검출할것을 권장합니다.

MYH15 항체 (C-8): sc-515748

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데이터 시트

    • 안티-MYH15 항체 C-8 는 마우스 monoclonal IgG1 (kappa light chain) MYH15 항체 이며 200 µg/ml으로 제공합니다.
    • 아미노 산 1068-1136 mapping within an internal region of MYH15 of human 의하고 기원한다
    • 안티-MYH15 항체 (C-8)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 MYH15 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-MYH15 항체 (C-8)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • MYH15 (C-8): sc-515748무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MYH15 Antibody (C-8) for WB applications. These reagents are now offered in bundles with MYH15 Antibody (C-8) (see ordering information below).
loading

How much sample do you recommend loading on the gel for Western blot detection using MYH15 (C-8): sc-515748?

Asked by: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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