MYH11 항체 (I-5) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 myosin heavy chain 11 isoforms SM-1 and SM-2을 검출할것을 권장합니다.

MYH11 항체 (I-5): sc-65734

MYH11 항체 (I-5) is rated 3.0 out of 5 by 3.
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데이터 시트

    • MYH11 항체(I-5) 는 마우스 monoclonal IgG1κ, 1간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against crude smooth muscle extract from uterus myometrium of human origin
    • 안티-MYH11 항체 (I-5)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 myosin heavy chain 11 isoforms SM-1 and SM-2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-MYH11 항체 (I-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • MYH11 (I-5): sc-65734무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MYH11 Antibody (I-5) for WB and IHC(P) applications. These reagents are now offered in bundles with MYH11 Antibody (I-5) (see ordering information below).
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How can MYH11 (I-5): sc-65734 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: AbPolly
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-65734 HRP), phycoerythrin (sc-65734 PE), fluoroscein (sc-65734 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 1 out of 5 by from Did not work on embryonic mouse tissue We used this antibody (1/100 AND 1/500) on paraformaldehyde fixed, paraffin embedded embryonic mouse tissue and we could not detect a specific signal.
Date published: 2018-06-05
Rated 4 out of 5 by from Works well for Western blot applications Works well for Western blot applications with HISM whole cell lysate. -SCBT QC
Date published: 2015-05-08
Rated 4 out of 5 by from Very strong band in WB with human uterine Very strong band in WB with human uterine fundus tissue extract. -SCBT QC
Date published: 2013-08-12
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