Mucin 16/MUC16 항체 (C-6) WB, IP, IF 와ELISA으로 human origin의 Mucin 16을 검출할것을 권장합니다.

Mucin 16/MUC16 항체 (C-6): sc-365002

Mucin 16/MUC16 항체 (C-6) is rated 5.0 out of 5 by 6.
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데이터 시트

    • 안티-Mucin 16/MUC16 항체 C-6 는 마우스 monoclonal IgG2a κ Mucin 16/MUC16 항체, 8간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Mucin 16에서 C-terminus에 위치한 6419-6568 아미노산을 항원으로 사용하였습니다.
    • Mucin 16/MUC16 항체 (C-6)는 WB, IP, IF and ELISA으로 human유래의 Mucin 16 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Mucin 16/MUC16 항체 (C-6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Mucin 16/MUC16 (C-6): sc-365002무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Mucin 16/MUC16 Antibody (C-6) for WB applications. These reagents are now offered in bundles with Mucin 16/MUC16 Antibody (C-6) (see ordering information below).
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Why did the Western blot analysis of mucin 16 show very different molecular weights in the whole lysate of MES-SA / Dx5, HeLa and MDA-MB-486. How was the lysate obtained for WB shown for sc-365002?

Asked by: DSteinbach
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
Thank you for your question We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2020-08-04
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Rated 5 out of 5 by from Great! This antibody works very well on cultured human cancer cells (immunofluorescence), definitely recommend!
Date published: 2018-04-15
Rated 5 out of 5 by from Look no further for great immunostaining! Beautiful cell surface localization by Immunofluorescence staining of methanol-fixed HeLa.
Date published: 2016-12-20
Rated 5 out of 5 by from Signal via western blot analysis of Mucin Signal via western blot analysis of Mucin 16 expression in MES-SA/Dx5 whole cell lysate. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Signal via immunofluorescence staining of methanol Signal via immunofluorescence staining of methanol-fixed HeLa cells showing cell surface localization. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Signal via immunofluorescence staining of methanol Signal via immunofluorescence staining of methanol-fixed HeLa cells showing cell surface localization.
Date published: 2015-03-14
Rated 5 out of 5 by from Signal via western blot analysis of Mucin Signal via western blot analysis of Mucin 16 expression in MDA-MB-468 whole cell lysate. -SCBT QC
Date published: 2013-08-14
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