LAT Antibody (LAT-01): sc-51663

LAT Antibody (LAT-01) is a mouse monoclonal IgG1 antibody that detects LAT in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). LAT, or linker for activation of T cells, is a crucial integral membrane protein that plays a significant role in T cell activation by serving as a signaling hub. Upon T cell receptor engagement, LAT associates with various signaling molecules, including phospholipase C gamma 1 (PLC γ1), GRB2, and the p85 subunit of phosphoinositide 3-kinase (PI 3-kinase), facilitating a cascade of intracellular signaling events that lead to T cell proliferation and differentiation. Notably, LAT undergoes palmitoylation, a post-translational modification that is essential for tyrosine phosphorylation and proper localization to glycolipid-enriched microdomains within the cell membrane. This localization is critical as LAT enhances the efficiency of signal transduction, ultimately influencing the activation of transcription factors such as AP-1 and NFAT, which are vital for the immune response. Anti-LAT antibody (LAT-01) is an invaluable tool for researchers studying T cell signaling pathways and their implications in immune responses.

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LAT Antibody (LAT-01) References:

1. Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxin.  |  Asazuma, N., et al. 2000. J Biol Chem. 275: 33427-34. PMID: 10942756
2. LAT, the linker for activation of T cells: a bridge between T cell-specific and general signaling pathways.  |  Wange, RL. 2000. Sci STKE. 2000: re1. PMID: 11752630
3. Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav.  |  Perez-Villar, JJ., et al. 2002. Biochemistry. 41: 10732-40. PMID: 12186560
4. Linker for activation of T cells integrates positive and negative signaling in mast cells.  |  Malbec, O., et al. 2004. J Immunol. 173: 5086-94. PMID: 15470052
5. Linker for activation of T cells contributes to airway inflammation in an asthmatic mouse model.  |  Guo, XJ., et al. 2010. Chin Med J (Engl). 123: 2676-81. PMID: 21034652
6. The importance of the Erk pathway in the development of linker for activation of T cells-mediated autoimmunity.  |  Fuller, DM., et al. 2012. J Immunol. 189: 4005-13. PMID: 22984075
7. The linker for activation of T cells (LAT) signaling hub: from signaling complexes to microclusters.  |  Balagopalan, L., et al. 2015. J Biol Chem. 290: 26422-9. PMID: 26354432
8. Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency.  |  Bacchelli, C., et al. 2017. J Allergy Clin Immunol. 139: 634-642.e5. PMID: 27522155
9. Protein 4.1R negatively regulates CD8+ T-cell activation by modulating phosphorylation of linker for activation of T cells.  |  Fan, D., et al. 2019. Immunology. 157: 312-321. PMID: 31135971
10. RNA-Sequencing Analysis and Expression of Lymphocyte-Specific Protein Tyrosine Kinase, Linker for Activation of T Cells, and 70-kDa T-Cell Receptor Zeta-Chain Associated Protein Kinase in Rat Liver Transplant Rejection.  |  Xuan, J., et al. 2023. Exp Clin Transplant. 21: 961-972. PMID: 38263783

The preferred LAT antibody is LAT Antibody (11B.12): sc-53550.