Laminin gamma-2 항체 (E-6) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Laminin γ-2을 검출할것을 권장합니다.

Laminin γ-2 항체 (E-6): sc-28330

Laminin γ-2 항체 (E-6) is rated 4.3 out of 5 by 7.
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데이터 시트

    • 안티-Laminin gamma-2 항체 E-6 는 마우스 monoclonal IgG1 κ Laminin γ-2 항체, 25간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Laminin γ-2에서 내부에 위치한 1011-1193 아미노산을 항원으로 사용하였습니다.
    • 안티-Laminin gamma-2 항체 (E-6)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Laminin γ-2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Laminin gamma-2 항체 (E-6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Laminin γ-2 (E-6): sc-28330무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Laminin γ-2 Antibody (E-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Laminin γ-2 Antibody (E-6) (see ordering information below).
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is this antibody suitable for IHC & IF against laminin 5 gamma 2?

Asked by: connectify
Thank you for your question. This antibody is recommended for the detection of Laminin γ-2 in many applications, including IF and IHC. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-08-30

I am using Laminin γ-2 (E-6): sc-28330 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not for us. We used this sample on paraffin embedded-formalin-fixed-bladder but did not work at all for us. We went as high as 10ug/ml and got no signal.
Date published: 2017-02-14
Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of Laminin γ-2 expression in A-431 whole cell lysate and mouse lung and rat lung tissue extracts.
Date published: 2017-01-27
Rated 5 out of 5 by from WB Published with this antibody using human WB Published with this antibody using human skin cell samples. -SCBT Publication Review
Date published: 2015-04-14
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lung carcinoma tissue. -SCBT QC
Date published: 2015-02-25
Rated 4 out of 5 by from Nice antibody to use for Western Blot Nice antibody to use for Western Blot, precise signal achieved in mouse lung and rat lung tissue extracts. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Publishable staining in paraffin Publishable staining in paraffin-embedded human esophagus tissue. -SCBT Publication Review
Date published: 2015-01-15
Rated 5 out of 5 by from IHC paraffin publishable data in normal human IHC paraffin publishable data in normal human fibroblasts, E10 cells, cancer tissue, and the collagen matrix containing fibroblasts. -SCBT Publication Review
Date published: 2015-01-12
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