Laminin-5 항체 (P3H9-2) WB, IP 와IF으로 human origin의 Laminin-5을 검출할것을 권장합니다.

Laminin-5 항체 (P3H9-2): sc-13586

Laminin-5 항체 (P3H9-2) is rated 4.2 out of 5 by 5.
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데이터 시트

    • 안티-Laminin-5 항체 P3H9-2 는 마우스 monoclonal IgG1 κ Laminin-5 항체, 15간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against human keratinocytes expressing laminin-5
    • Laminin-5 항체 (P3H9-2)는 WB, IP and IF으로 human유래의 Laminin-5 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Laminin-5 항체 (P3H9-2)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Laminin-5 (P3H9-2): sc-13586무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Laminin-5 Antibody (P3H9-2) for WB applications. These reagents are now offered in bundles with Laminin-5 Antibody (P3H9-2) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with Laminin-5 (P3H9-2): sc-13586 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Does not work in IF This antibody does not work in IF for Caco-2 cells.
Date published: 2018-05-10
Rated 5 out of 5 by from Good IF data with human skin samples Good IF data with human skin samples. -SCBT Publication Review
Date published: 2015-04-10
Rated 5 out of 5 by from Publishable immunostaining in paraffin embedded Publishable immunostaining in paraffin embedded human colorectal adenocarcinoma. -SCBT Publication Review
Date published: 2015-03-15
Rated 5 out of 5 by from IF data published in human foreskin samples IF data published in human foreskin samples. -SCBT Publication Review
Date published: 2015-01-19
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmic Exceptional immunofluorescence cytoplasmic staining in methanol-fixed A-431 cells. -SCBT QC
Date published: 2015-01-15
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