KIR4.2 항체 (G-5) WB, IP, IF 와ELISA으로 human origin의 KIR4.2을 검출할것을 권장합니다.

KIR4.2 항체 (G-5): sc-376322

KIR4.2 항체 (G-5) is rated 5.0 out of 5 by 3.
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데이터 시트

    • 안티-KIR4.2 항체 G-5 는 마우스 monoclonal IgG2a (kappa light chain) KIR4.2 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 KIR4.2을 항원으로 사용하였습니다.
    • KIR4.2 항체 (G-5)는 WB, IP, IF and ELISA으로 human유래의 KIR4.2 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-KIR4.2 항체 (G-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • KIR4.2 (G-5): sc-376322무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for KIR4.2 Antibody (G-5) for WB applications. These reagents are now offered in bundles with KIR4.2 Antibody (G-5) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with KIR4.2 (G-5): sc-376322 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Good signal in flow cytometry I used this antibody on human HL-60 cells in flow cytometry. Cells were fixed in 4% PFA in PBS then permeabilized with 0.1% TritonX-100. Good signal was achieved using 1:200 antibody dilution.
Date published: 2021-03-18
Rated 5 out of 5 by from Exceptional immunofluorescence membrane staining Exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Satisfactory Western Blot data of KIR4 Satisfactory Western Blot data of KIR4.2 expression in Hep G2 and HeLa whole cell lysates. -SCBT QC
Date published: 2013-03-31
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