HNF-3 beta 항체 (H-8) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 HNF-3β을 검출할것을 권장합니다.

HNF-3β 항체 (H-8): sc-271104

HNF-3β 항체 (H-8) is rated 5.0 out of 5 by 2.
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데이터 시트

    • HNF-3β 항체(H-8) 는 마우스 monoclonal IgG2bκ, 1간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 261-370 mapping within an internal region of HNF-3β of human origin
    • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 HNF-3β을 검출할것을 권장합니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-271104 X, 200 µg/0.1 ml)이 있습니다.
    • See HNF-3β (H-4): sc-374376 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for HNF-3β Antibody (H-8) for WB applications. This reagent is now offered in a bundle with HNF-3β Antibody (H-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended fixation method for immunofluorescence staining with HNF-3β (H-8): sc-271104 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Fuzzy but strong bands seen in Western Blot Fuzzy but strong bands seen in Western Blot using Hep G2 and A549 nuclear extracts -SCBT QC
Date published: 2015-01-24
Rated 5 out of 5 by from Great for PFA fixed IF with Hep G2 cells Great for PFA fixed IF with Hep G2 cells -SCBT QC
Date published: 2013-09-22
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