HNF-3 beta 항체 (B-2) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 HNF-3β을 검출할것을 권장합니다.

HNF-3β 항체 (B-2): sc-271103

HNF-3β 항체 (B-2) is rated 4.8 out of 5 by 4.
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데이터 시트

    • HNF-3β 항체(B-2) 는 마우스 monoclonal IgG1κ, 4간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 261-370 mapping within an internal region of HNF-3β of human origin
    • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 HNF-3β을 검출할것을 권장합니다.
    • ChIP 에 사용하는 TransCruz시약(sc-271103 X, 200 µg/0.1 ml)이 있습니다.
    • See HNF-3β (H-4): sc-374376 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HNF-3β Antibody (B-2). This work is in progress.
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What is the recommended fixation method for immunofluorescence staining with HNF-3β (B-2): sc-271103 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great Antibody for IF This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Beautiful IF staining.
Date published: 2017-01-23
Rated 4 out of 5 by from Our lab tried this antibody in IF and got Our lab tried this antibody in IF and got good staining pattern.
Date published: 2015-09-08
Rated 5 out of 5 by from Strong nuclear localization seen in IF with Strong nuclear localization seen in IF with HepG2 cells -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by from Good results in WB with Hep G2 and A549 nuclear Good results in WB with Hep G2 and A549 nuclear extracts. -SCBT QC
Date published: 2014-02-15
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