HNF-3 beta 항체 (A-12) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 HNF-3β을 검출할것을 권장합니다.

HNF-3β 항체 (A-12): sc-374375

HNF-3β 항체 (A-12) is rated 5.0 out of 5 by 4.
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데이터 시트

    • HNF-3β 항체(A-12) 는 마우스 monoclonal IgG1κ, 6간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 mouse origin의 HNF-3β의 내부에 위치한 431-459의 아미노산에 해당됩니다.
    • 안티-HNF-3 beta 항체 (A-12)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 HNF-3β 를 감지하는 데에 추천한다.; 이외에, equine, canine, bovine and porcine등 species와 반응할수 있습니다
    • ChIP 에 사용하는 TransCruz시약(sc-374375 X, 200 µg/0.1 ml)이 있습니다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-HNF-3 beta 항체 (A-12)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • HNF-3β (A-12): sc-374375무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for HNF-3β Antibody (A-12) for WB applications. This reagent is now offered in a bundle with HNF-3β Antibody (A-12) (see ordering information below).
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What application is the blocking peptide sc-374375 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from broad species reactivity The (A-12) clone is a good choice for an HNF-3beta antibody in a broad range of species including mouse, rat, human and additional species reactivity in equine, bovine, canine and porcine. However, we do offer some alternative clones which have stronger in-house data including HNF-3β (B-2): sc-271103.
Date published: 2017-02-02
Rated 5 out of 5 by from Strong nuclear localization and high fluorescence Strong nuclear localization and high fluorescence expression with low concentration.
Date published: 2015-10-16
Rated 5 out of 5 by from Great for IF with PFA fixed HepG2 cells Great for IF with PFA fixed HepG2 cells - good nuclear localization -SCBT QC
Date published: 2015-01-25
Rated 5 out of 5 by from Positive bands seen in Hep G2 and A549 nuclear Positive bands seen in Hep G2 and A549 nuclear extracts in Western Blot -SCBT QC
Date published: 2014-06-10
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