항원결정부는 human origin의 HNF-3α의 내부에 위치한 445-473의 아미노산에 해당됩니다.
안티-HNF-3 alpha / beta 항체 (E-4)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 HNF-3α and HNF-3β 를 감지하는 데에 추천한다.; 이외에, bovine and porcine등 species와 반응할수 있습니다
ChIP 에 사용하는 TransCruz시약(sc-377033 X, 200 µg/0.1 ml)이 있습니다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-HNF-3 alpha / beta 항체 (E-4)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgGκ BP-HRP is the preferred secondary detection reagent for HNF-3α/β Antibody (E-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with HNF-3α/β Antibody (E-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
HNF-3α/β Antibody (E-4) is a high quality monoclonal HNF-3 alpha / beta antibody (also designated FOXA2 antibody, HNF3B antibody or forkhead box A2 antibody) suitable for the detection of the HNF-3 alpha / beta protein of mouse, rat and human origin. HNF-3α/β Antibody (E-4) is available as both the non-conjugated anti-HNF-3 alpha / beta antibody form, as well as multiple conjugated forms of anti-HNF-3 alpha / beta antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation. HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4α is expressed in liver, kidney, pancreas, small intestine, testis and colon; HNF-4γ is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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