HNF-1 alpha 항체 (F-7) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 HNF-1α을 검출할것을 권장합니다.

HNF-1α 항체 (F-7): sc-393925

HNF-1α 항체 (F-7) is rated 4.3 out of 5 by 7.
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데이터 시트

    • 안티-HNF-1 alpha 항체 F-7 는 마우스 monoclonal IgG2a κ HNF-1α 항체, 4간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 HNF-1α에서 내부에 위치한 80-284 아미노산을 항원으로 사용하였습니다.
    • 안티-HNF-1 alpha 항체 (F-7)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 HNF-1α 를 감지하는 데에 추천한다.; 이외에, canine and porcine등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-HNF-1 alpha 항체 (F-7)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-393925 X, 200 µg/0.1 ml)이 있습니다.
    • HNF-1α (F-7): sc-393925무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HNF-1α Antibody (F-7). This work is in progress.
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Can HNF-1α (F-7): sc-393925 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HNF-1α (F-7): sc-393925 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 抗体不错 本人半月前申请的HNF-1a小样试用装,跑了蛋白,条带不错,推荐使用,另外,有现货的话,基本一天到货,赞
Date published: 2017-09-22
Rated 4 out of 5 by from Good The antibody works well, especially after overnight incubation. I used at 1:1000 dilution.The band is strong and clear,the background is also clear.
Date published: 2017-07-14
Rated 5 out of 5 by from Great has been the antibody, than we have to buy t Salesman to apply for HBF1a antibodies, used, and very good use of this protein before the Abcam has not been used to do the experiment, although it is a trial installation, but better than before.
Date published: 2017-01-07
Rated 1 out of 5 by from Unsuitable for IHC(P) Unsuitable for IHC(P). Unspecifically stains confirmed HNF1A negative samples at various dilutions, no difference between HNF1A positive and negative samples. Cytoplasmic instead of nuclear staining. Not an alternative for sc-8986 for this application.
Date published: 2016-08-16
Rated 5 out of 5 by from I used I used, it is good to use, than before to buy some of the antibodies are actually better use
Date published: 2016-08-02
Rated 5 out of 5 by from Very good quality on Rat Liver tissue in WB Very good quality on Rat Liver tissue in WB.we tried 1:500 to 1:100. The optimize dilution is 1:300. I use lot:B0514. I give full score of this antibody. I'll order again.
Date published: 2015-09-09
Rated 5 out of 5 by from Great results in Western Blot with Hep G2 Great results in Western Blot with Hep G2 whole cell lysate and human liver and rat liver tissue extracts -SCBT QC
Date published: 2014-02-15
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