HNF-1 alpha 항체 (2) WB, IP와 IF으로 mouse, rat와 human origin의 HNF-1α을 검출할것을 권장합니다.

HNF-1α 항체 (2): sc-135939

HNF-1α 항체 (2) is rated 4.0 out of 5 by 3.
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데이터 시트

    • HNF-1 alpha 항체 2 는 마우스 monoclonal IgG1, 5간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • mouse origin의 HNF-1α에서 내부에 위치한 70-269 아미노산을 항원으로 사용하였습니다.
    • WB, IP와 IF으로 mouse, rat와 human origin의 HNF-1α을 검출할것을 권장합니다.
    • See HNF-1α (F-7): sc-393925 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HNF-1α Antibody (2) for WB applications. These reagents are now offered in bundles with HNF-1α Antibody (2) (see ordering information below).
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I am using HNF-1α (2): sc-135939 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good antibody Works as we expected. Tested by immunofluorescence.
Date published: 2018-12-18
Rated 4 out of 5 by from Clean band seen in WB with rat liver tissue Clean band seen in WB with rat liver tissue extract. -SCBT QC
Date published: 2015-02-21
Rated 4 out of 5 by from Good nuclear staining of endothelial cells Good nuclear staining of endothelial cells seen in IF -SCBT QC
Date published: 2013-04-28
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