GATA-1 항체 (N6) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 GATA-1을 검출할것을 권장합니다.

GATA-1 항체 (N6): sc-265

GATA-1 항체 (N6) is rated 4.5 out of 5 by 16.
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데이터 시트

    • 안티-GATA-1 항체 N6 는 랫드 monoclonal IgG2a GATA-1 항체, 288간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • mouse origin의 GATA-1의 전체인 아미노산을 항원으로 사용하였습니다.
    • 안티-GATA-1 항체 (N6)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 GATA-1 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-GATA-1 항체 (N6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-265 X, 200 µg/0.1 ml)이 있습니다.
    • GATA-1 (N6): sc-265무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

Is sc-265 X also suitable for Western blotting?

Asked by: Kiki Chem
Thank you for your question. Yes, you can use sc-265 X for Western blotting. The only difference is that the "X" version of the antibody is 10x more concentrated in a buffer without gelatin (PBS + sodium azide only). Therefore, we recommend adjusting the recommended dilutions for Western blotting accordingly. For example, instead of using a 1:100 dilution, you could use a 1:1,000 dilution for Western blotting with the sc-265 X version of the antibody.
Answered by: Technical Support
Date published: 2020-05-07

Does GATA-1 (N6) sc-265 antibody recognize the full and short isoform of GATA-1?

Asked by: Ricko
Thank you for your question. GATA-1 Antibody (N6) is a rat monoclonal IgG2a raised against recombinant GATA-1 of mouse origin. The exact epitope recognized by this monoclonal antibody has not been determined. We have not specifically tested this antibody for reactivity with different isoforms of GATA-1. We recommend you refer to some of the product citations using this antibody to see if anyone else has published this information previously.
Answered by: Technical Support
Date published: 2019-12-03

Does GATA-1 (N6) sc-265 bind to N-terminal or C-terminal of GATA1? I want to detect N-terminal only. Thank you.

Asked by: Jesmine
Thank you for your question. This antibody, sc-265 GATA-1 (N6), maps to the N-terminus of GATA-1. If you have any further questions, please reach out to your local distributor. You can find their information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-12-04

What is the recommended storage condition for GATA-1 (N6): sc-265?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from 免疫組織化学で良好な結果 免疫蛍光染色により精巣におけるセルトリ細胞のGATA-1を検出できた。 さらに、特異性も高く優れた抗体である。
Date published: 2021-01-29
Rated 4 out of 5 by from 抗体小样很给力 买了一支常用抗体,赠送的小样,很慷慨,很好用。建议多搞这样的活动,有些分子不确定表达,就是试一把。这时候小样很重要。确定表达了,再买,减少浪费
Date published: 2020-06-28
Rated 5 out of 5 by from Great Images! Used it in an EMSA setting to detect GATA-1 binding to DNA and got clear data. Totaly recommended!
Date published: 2019-10-05
Rated 5 out of 5 by from IF很好用 我用在小鼠卵黄膜上血细胞的免疫荧光染色,1:200稀释很好用,背景很低,阳性信号强,是一个好抗体,推荐!
Date published: 2018-10-18
Rated 4 out of 5 by from Works First purchased this antibody in 2012 and 3 years later, it stopped working despite different antigen retrieval methods. Many SC antibodies work well beyond their recommended use date, but this is not one of them. So re-ordered in 2018 and it works well. We used this antibody on PFA fixed, paraffin embedded mouse testis cut at 5um sections, for immuno-fluorescent staining. We used a pH6 antigen retrieval method in a PT-Link machine or pressure cooker. NOTE: The antibody does not work on bouin fixed tissues.
Date published: 2018-06-05
Rated 5 out of 5 by from Good for WB, Co-IP, and ChIP This antibody is good for Western blot, co-IP and ChIP using mouse cells.
Date published: 2018-05-12
Rated 5 out of 5 by from GATA-1 antibody I used the antibody for immunofluroscence staining and the antibody worked fine
Date published: 2017-09-22
Rated 5 out of 5 by from Superb antibody Clear, specific western along with very strong ihc data
Date published: 2017-01-03
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