Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
DND1 Antibody (X22) is a high quality monoclonal DND1 antibody (also designated DND1 antibody) suitable for the detection of the DND1 protein of mouse, rat and human origin. DND1 Antibody (X22) is available as the non-conjugated anti-DND1 antibody. Primordial germ cells (PGCs) are precursor germ cells that divide rapidly and migrate toward the developing gonads. DND1 (dead end protein homolog 1), also known as RBMS4 (RNA-binding motif, single-stranded-interacting protein 4), is a 353 amino acid protein that contains two RRM (RNA recognition motif) domains and localizes to perinuclear germ granules within PGCs. Expressed specifically in PGCs throughout embryogenesis, DND1 is thought to play a role in PGC development, but is not necessary for PGC migration to gonadal regions. Mutations in the gene encoding DND1 may result in germ cell loss and the subsequent development of testicular germ cell tumors, suggesting that DND1 may play a role in tumorigenesis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using DND1 (X22): sc-130493 antibody for immunoprecipitation, how much antibody should I use?
Asked by: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: