DGAT1 항체 (A-5) WB, IP, IF, IHC(P) 와ELISA으로 human origin의 DGAT1을 검출할것을 권장합니다.

DGAT1 항체 (A-5): sc-271934

DGAT1 항체 (A-5) is rated 5.0 out of 5 by 4.
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데이터 시트

    • 안티-DGAT1 항체 A-5 는 마우스 monoclonal IgG1 κ DGAT1 항체, 11간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 DGAT1을 항원으로 사용하였습니다.
    • DGAT1 항체 (A-5)는 WB, IP, IF, IHC(P) and ELISA으로 human유래의 DGAT1 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-DGAT1 항체 (A-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • DGAT1 (A-5): sc-271934무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG1 BP-HRP is the preferred secondary detection reagent for DGAT1 Antibody (A-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with DGAT1 Antibody (A-5) (see ordering information below).
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What is the difference between DGAT1 (A-5) and DGAT1 (G-6) antibody? Which one do you recommend for WB with Huh 7.5 cell?

Asked by: Katsuki
Thank you for your question. These are two different mouse monoclonal antibodies both suitable for Western blot of human DGAT1. They are different isotypes and have different epitopes. We have not tested either antibody in Huh 7.5 cells.
Answered by: Technical Support
Date published: 2018-11-30

Can it be used for WB with Huh 7.5 cells?

Asked by: Katsuki
Thank you for your question. This antibody, DGAT1 (A-5): sc-271934 , is recommended for Western blot. We have not tested the antibody specifically on Huh 7.5 cells.
Answered by: Technical Support
Date published: 2018-11-30

Can DGAT1 (A-5): sc-271934 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, DGAT1 (A-5): sc-271934 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from good resources use it at 1:200 for WB ,get a good resource is singer ana clear.
Date published: 2017-08-18
Rated 5 out of 5 by from Worked well for western blotting with human Worked well for western blotting with human DGAT1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-23
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from WB: clean bands in HeLa and Hep G2 whole WB: clean bands in HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-03-09
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