안티-cyclin H 항체 D-10 는 마우스 monoclonal IgG2b κ cyclin H 항체, 13간행물에 인용, 이며 200 µg/ml으로 제공합니다.
full length cyclin H of human 의하고 제작된다
안티-cyclin H 항체 (D-10)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 cyclin H p37 (CAK regulatory subunit) 를 감지하는 데에 추천한다.; catalytic subunit, (Cdk7)와 교차반응이 없습니다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin H 항체 (D-10)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG2b BP-HRP is the preferred secondary detection reagent for cyclin H Antibody (D-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin H Antibody (D-10) (see ordering information below).
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cyclin H Antibody (D-10) is a high quality monoclonal cyclin H antibody (also designated Cyclin H/p34 antibody) suitable for the detection of the cyclin H protein of mouse, rat and human origin. cyclin H Antibody (D-10) is available as both the non-conjugated anti-cyclin H antibody form, as well as multiple conjugated forms of anti-cyclin H antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires, in addition, phosphorylation at Thr 160. The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated earlier in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine required for full activity; mutation of this residue severely reduces CAK activity.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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