cyclin H 항체 (D-10) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 cyclin H p37 (CAK regulatory subunit)을 검출할것을 권장합니다.

cyclin H 항체 (D-10): sc-1662

cyclin H 항체 (D-10) is rated 4.8 out of 5 by 5.
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데이터 시트

    • 안티-cyclin H 항체 D-10 는 마우스 monoclonal IgG2b κ cyclin H 항체, 13간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • full length cyclin H of human 의하고 제작된다
    • 안티-cyclin H 항체 (D-10)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 cyclin H p37 (CAK regulatory subunit) 를 감지하는 데에 추천한다.; catalytic subunit, (Cdk7)와 교차반응이 없습니다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin H 항체 (D-10)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • IF, IHC(P) 와 FCM에 사용가능한 TRITC 결합제품이 있습니다.
    • cyclin H (D-10): sc-1662무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for cyclin H Antibody (D-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin H Antibody (D-10) (see ordering information below).
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Can cyclin H (D-10): sc-1662 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin H (D-10): sc-1662 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from good for western In HeLa, it is a fairly clean, but very low-affinity antibody. Several hour exposure needed at even at 1:250 dilution. Would work well for cyclin-H overexpression.
Date published: 2017-04-12
Rated 5 out of 5 by from Good staining with paraffin Good staining with paraffin-embedded human breast samples. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in A-431. -SCBT QC
Date published: 2015-01-23
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in Jurkat, HeLa, A-431, C32, MM-142 and NIH/3T3. -SCBT QC
Date published: 2014-01-14
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