cyclin E 항체 (HE12) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 cyclin E (doublet at 50 kDa and single band at 42 kDa)을 검출할것을 권장합니다.

cyclin E 항체 (HE12): sc-247

cyclin E 항체 (HE12) is rated 4.7 out of 5 by 19.
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데이터 시트

    • cyclin E 항체(HE12) 는 마우스 monoclonal IgG1κ, 769간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 cyclin E의 전체인 아미노산을 항원으로 사용하였습니다.
    • 안티-cyclin E 항체 (HE12)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 cyclin E (doublet at 50 kDa and single band at 42 kDa) 를 감지하는 데에 추천한다.; Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • IF, IHC(P) and FCM를 위해FITC 에 결합된 cyclin E 항체 (HE12)를 제공한다.
    • IF, IHC(P) and FCM에 사용하는Alexa Fluor® 594 접합 제품도 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • cyclin E (HE12): sc-247무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin E Antibody (HE12) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin E Antibody (HE12) (see ordering information below).
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Which buffer to use for western blot? I used 5% milk in TBS-T for blocking and for both primary and secondary Ab and got no band. Thanks

Asked by: Yaelle
Thank you for your question. We recommend using 5% nonfat milk in TBST for blocking and for both primary and secondary antibody incubations. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-11-25

Is the antibody directed against the whole protein or any particular site (c-or n-terminal?)

Asked by: portland
Thank you for your question. This antibody was made against a recombinant synthetic peptide corresponding to Human Cyclin E1. The exact epitope has not been epitope mapped, so I do not have further information regarding any particular site. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2018-02-07

Can the antibody used on both rat and mouse source samples? How long will it take to get the antibody?

Asked by: Guanwen
Thank you for your question. cyclin E Antibody (HE12): sc-247 is recommended for detection of cyclin E in mouse, rat and human samples. Please contact our Asian Support Office to inquire about current product availability and shipping and delivery times for your institution. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-04-07

Does it recognize both CCNE1 and CCNE2? thanks!

Asked by: NickNR
Thank you for your question. cyclin E Antibody (HE12): sc-247 is recommended for detection of cyclin E (CCNE1). We have not tested the antibody for reactivity against CCNE2.
Answered by: Technical Support
Date published: 2017-03-30

Is it possible to use this mouse monoclonal antibody to detect cyclin E in canine samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on our website, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your desired species.
Answered by: Tech Service 8
Date published: 2016-12-16

Can I use the cyclin E Antibody (HE12) for immunofluorescence if I am working with mouse tissue samples?

Asked by: Trav11
If looking to perform immunofluorescence in mouse tissue samples, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the cyclin E Antibody (HE12): sc-247 is available conjugated to fluorescein (sc-247 FITC, 200 µg/ml) and TRITC (sc-247 TRITC, 200 µg/ml).
Answered by: Tech Service 8
Date published: 2016-12-16
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Rated 5 out of 5 by from western 没有申请小样,直接买的大包装抗体,用起来相当不错,K562细胞制备的蛋白样品。所以强烈推荐,毕竟性价比还是不错的!
Date published: 2020-12-20
Rated 5 out of 5 by from Good antibody for WB I used this antibody in western blot analysis obtaining a clear signal at the predicted position
Date published: 2020-09-08
Rated 5 out of 5 by from good product It is a good antibody with clean background for WB.
Date published: 2018-09-11
Rated 5 out of 5 by from Very strong signal Sharp and strong band in westen blot carried out on samples derived from human primary macrophages.
Date published: 2018-03-12
Rated 4 out of 5 by from HE12, Santa Cruz: a good antibody This antibody works really well and for this reason we choose it among all the other that we had the possibility to test.
Date published: 2018-01-18
Rated 5 out of 5 by from Good antibody! 1:200 diluted for wb of rat heart protein,the band is strong and special
Date published: 2017-12-30
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Strong signal I used cyclin E (HE12) antibody (1:500) dilution to check S phase in cell cycle experiment and got a strong signal and I can see a difference among my samples.
Date published: 2017-08-25
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