cyclin E 항체 (HE111) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 cyclin E을 검출할것을 권장합니다.

cyclin E 항체 (HE111): sc-248

cyclin E 항체 (HE111) is rated 4.8 out of 5 by 6.
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데이터 시트

    • cyclin E 항체 (HE111) 는 마우스 단클론 IgG1 λ, 133간행물에 인용,이며 200 µg/ml로 제공합니다.
    • human origin의 cyclin E의 전체인 아미노산을 항원으로 사용하였습니다.
    • 안티-cyclin E 항체 (HE111)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 cyclin E 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin E 항체 (HE111)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • IF, IHC(P) 와 FCM에 사용가능한 TRITC 결합제품이 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-248 X, 200 µg/0.1 ml)이 있습니다.
    • cyclin E (HE111): sc-248무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin E Antibody (HE111). This work is in progress.
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Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from mouse liver nice antibody 1:500 dilution, in mouse liver tissue.
Date published: 2017-03-31
Rated 5 out of 5 by from Great IHC results Tested multiple samples by IHC in paraffin embedded sections with great results!
Date published: 2017-01-17
Rated 5 out of 5 by from Good data with paraffin Good data with paraffin-embedded human ovary tissue. -SCBT Publication Review
Date published: 2015-02-11
Rated 4 out of 5 by from This antibody works well and can be used This antibody works well and can be used for IP with MEG-01 samples. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from WB: Great antibody WB: Great antibody, good images for publication with human U2OS. -SCBT Publication Review
Date published: 2014-08-20
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