cyclin E 항체 (HE111) 는 마우스 단클론 IgG1 λ, 133간행물에 인용,이며 200 µg/ml로 제공합니다.
human origin의 cyclin E의 전체인 아미노산을 항원으로 사용하였습니다.
안티-cyclin E 항체 (HE111)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 cyclin E 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin E 항체 (HE111)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
IF, IHC(P) 와 FCM에 사용가능한 TRITC 결합제품이 있습니다.
Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-248 X, 200 µg/0.1 ml)이 있습니다.
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Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. Cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.
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