안티-cyclin E 항체 E-4 는 마우스 monoclonal IgG2b κ cyclin E 항체, 84간행물에 인용, 이며 200 µg/ml으로 제공합니다.
항원결정부는 rat origin의 cyclin E의 내부에 위치한 367-396의 아미노산에 해당됩니다.
안티-cyclin E 항체 (E-4)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 cyclin E1 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin E 항체 (E-4)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin E Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin E Antibody (E-4) (see ordering information below).
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cyclin E Antibody (E-4) is a high quality monoclonal cyclin E antibody (also designated CCNE1 antibody) suitable for the detection of the cyclin E protein of mouse, rat and human origin. cyclin E Antibody (E-4) is available as both the non-conjugated anti-cyclin E antibody form, as well as multiple conjugated forms of anti-cyclin E antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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