cyclin E 항체 (B-7) WB, IP, IF 와IHC(P)으로 human origin의 cyclin E을 검출할것을 권장합니다.

cyclin E 항체 (B-7): sc-48420

cyclin E 항체 (B-7) is rated 4.0 out of 5 by 1.
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데이터 시트

    • cyclin E 항체(B-7) 는 마우스 monoclonal IgG2aκ, 10간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 cyclin E에서 내부에 위치한 1-145 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF 와IHC(P)으로 human origin의 cyclin E을 검출할것을 권장합니다.
    • See cyclin E (E-4): sc-377100 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for cyclin E Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin E Antibody (B-7) (see ordering information below).
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I am using cyclin E (B-7): sc-48420 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Reasonably specific and sensitive in WB with K Reasonably specific and sensitive in WB with K-562 and Jurkat nuclear extracts. -SCBT QC
Date published: 2014-02-01
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