cyclin D2 항체 (DCS-5) WB, IP, IF, FCM와 ELISA으로 mouse, rat와 human origin의 cyclin D2을 검출할것을 권장합니다.

cyclin D2 항체 (DCS-5): sc-53637

cyclin D2 항체 (DCS-5) is rated 4.5 out of 5 by 6.
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데이터 시트

    • cyclin D2 항체(DCS-5) 는 마우스 monoclonal IgG2bκ, 12간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • full length cyclin D2 of human 의하고 제작된다
    • 안티-cyclin D2 항체 (DCS-5)는 WB, IP, IF, FCM and ELISA으로 mouse, rat and human유래의 cyclin D2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin D2 항체 (DCS-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • cyclin D2 (DCS-5): sc-53637무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for cyclin D2 Antibody (DCS-5) for WB applications. These reagents are now offered in bundles with cyclin D2 Antibody (DCS-5) (see ordering information below).
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Can cyclin D2 (DCS-5): sc-53637 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin D2 (DCS-5): sc-53637 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Specific Western detection in rat INS-1 cells 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong, specific band of ~38 kD. Antibody sc-56305 is also suitable. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 5 out of 5 by from Nice signal by WB on mouse colon extracts This antibody works perfectly on mouse colon extracts (20 ug) at a 1/200 dilution with regular ECL.
Date published: 2017-10-27
Rated 4 out of 5 by from a relative good antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). However, I would recommend to use sc-376676 for WB.
Date published: 2017-09-18
Rated 3 out of 5 by from not very clean In HeLa, it is a low-affinity antibody. Not very clean, it has many nonspecific larger MW bands which are stronger than the cyclin-D2 band.
Date published: 2017-04-12
Rated 5 out of 5 by from Mono works in human transfected lysates Mono works in human transfected lysates. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Works well in MM Works well in MM-142 nuclear extract under reducing and non-reducing conditions. -SCBT QC
Date published: 2014-07-17
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