cyclin D2 항체 (DCS-3) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 cyclin D2을 검출할것을 권장합니다.

cyclin D2 항체 (DCS-3): sc-56305

cyclin D2 항체 (DCS-3) is rated 5.0 out of 5 by 4.
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데이터 시트

    • cyclin D2 항체(DCS-3) 는 마우스 monoclonal IgG2aκ, 8간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • full length cyclin D2 of human 의하고 제작된다
    • 안티-cyclin D2 항체 (DCS-3)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 cyclin D2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin D2 항체 (DCS-3)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • cyclin D2 (DCS-3): sc-56305무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for cyclin D2 Antibody (DCS-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin D2 Antibody (DCS-3) (see ordering information below).
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I am using cyclin D2 (DCS-3): sc-56305 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Specific Western detection in rat INS-1 cells 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a specific, moderately strong band of ~38 kD. Can also use sc-53637. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 5 out of 5 by from Popular choice for WB! The (DCS-3) clone is a popular choice for Western blotting. It has been featured in at least four publications in the literature for this application.
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent antibody for WB with MM Excellent antibody for WB with MM-142. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Robust staining in paraffin Robust staining in paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2014-12-17
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