cyclin D1 항체 (HD11) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 cyclin D1을 검출할것을 권장합니다.

cyclin D1 항체 (HD11): sc-246

cyclin D1 항체 (HD11) is rated 4.4 out of 5 by 16.
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데이터 시트

    • cyclin D1 항체 HD11 는 마우스 monoclonal IgG1, 400간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 cyclin D1의 전체인 아미노산을 항원으로 사용하였습니다.
    • 안티-cyclin D1 항체 (HD11)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 cyclin D1 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin D1 항체 (HD11)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • cyclin D1 (HD11): sc-246무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin D1 Antibody (HD11). This work is in progress.
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Hi, I ve just used the antibody sc-246 for mouse tissue and I am getting two different bands. One at around 50kDa and another one at around 25kDa. Can I accept one of these as mouse Cyclin D1 ? Thank you in advance.

Asked by: ElliPapas
Dear Ellipapas, Thank you for your question. We want you to be successful in your research. To help you, i need more Information about your Protocol. Please contact our European Support office by phone: toll-free 00800 4573 8000, email [email protected] or live chat. We are happy to help you to troubleshoot the experiment.
Answered by: Technical Support Europe
Date published: 2017-08-15

I would like to use the cyclin D1 Antibody (HD11): sc-246 for IHC(P) in mouse tissue. Is this possible?

Asked by: cjMara
Thank you for your question. If looking to use a mouse monoclonal primary antibody for IHC(P) in mouse tissue, we recommend using our directly conjugated primary antibodies. Currently, sc-246 is not available with direct conjugation, but an alternative cyclin D1 antibody, sc-8396, is available with conjugation to agarose, HRP, FITC, PE, Alexa Fluor 488 and Alexa Fluor 647. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-22

I would like to use the cyclin D1 Antibody (HD11): sc-246 for IHC(P) in mouse tissue. Is this possible?

Asked by: Trav11
Thank you for your question. If looking to use a mouse monoclonal primary antibody for IHC(P) in mouse tissue, we recommend using our directly conjugated primary antibodies. Currently, sc-246 is not available with direct conjugation, but an alternative cyclin D1 antibody, sc-8396, is available with conjugation to agarose, HRP, FITC, PE, Alexa Fluor 488 and Alexa Fluor 647. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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Rated 5 out of 5 by from Good for WB It is useful for western blot analysis of human cell line.
Date published: 2020-09-04
Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysates Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Recommend sc-20044 instead
Date published: 2019-05-22
Rated 4 out of 5 by from weak signal very weak signal on Hela cell lysate with some background
Date published: 2018-08-22
Rated 4 out of 5 by from GOOD ANTIBODY I got a sample of this sample as a free sample and it is working quite good. I have plans to buy this antibody as soon as possible
Date published: 2018-03-29
Rated 4 out of 5 by from Specific binding I bought this 5 years back during my PhD program for our Molecular Medicine laboratory in India. This antibody has good specificity to cell lysates of human origin. It worked well with a dilution of 1:2500 and the bands were clearly visible through ECL detection even after 3 to 4 reuses of the diluted antibody.
Date published: 2017-06-22
Rated 4 out of 5 by from Good single band Good clear band. Little to no background. Specificity on point!
Date published: 2017-06-07
Rated 5 out of 5 by from Used in IHC Worked well at 1:100 dilution for IHC on rat brain sections, very happy.
Date published: 2017-04-07
Rated 5 out of 5 by from Strong western data Very specific western blot results; strong clear signal
Date published: 2017-01-30
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