cyclin B 항체 (D-1) WB, IP, IF 와ELISA으로 d origin의 cyclin B을 검출할것을 권장합니다.

cyclin B 항체 (D-1): sc-166210

cyclin B 항체 (D-1) is rated 4.1 out of 5 by 9.
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데이터 시트

    • 안티-cyclin B 항체 D-1 는 마우스 monoclonal IgG1 κ cyclin B 항체, 13간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • Drosophila melanogaster origin의 cyclin B에서 내부에 위치한 1-300 아미노산을 항원으로 사용하였습니다.
    • cyclin B 항체 (D-1)는 WB, IP, IF and ELISA으로 d유래의 cyclin B 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin B 항체 (D-1)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • cyclin B (D-1): sc-166210무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin B Antibody (D-1) for WB applications. These reagents are now offered in bundles with cyclin B Antibody (D-1) (see ordering information below).
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What blocking solution should I use with cyclin B (D-1): sc-166210 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 5 out of 5 by from Very specific antibody This antibody works well with my project! No non-specific bands.
Date published: 2020-02-24
Rated 4 out of 5 by from Good features Good quality antibody for western blot detection of cyclin B in cell lysates with visible difference between proliferating and growth arrested cells
Date published: 2018-03-12
Rated 3 out of 5 by from an OK antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), albeit the intensity is somewhat weak
Date published: 2017-09-18
Rated 4 out of 5 by from not suitable for turbot there is a very light band when i exposed for 25min
Date published: 2017-07-10
Rated 1 out of 5 by from Didnot work for WB No band was observed in MCF-7 cell lysates. Used the ab at 1:1000 dilution.
Date published: 2017-07-03
Rated 5 out of 5 by from Great feature This antibody has been tested by WB for HepG2 cells. I used the product and diluted in TBST(1:200),4℃ overnight.
Date published: 2017-06-02
Rated 5 out of 5 by from show good result In my Western blot analysis,this cyclinB antibody show good performance.The band is very strong.
Date published: 2016-12-05
Rated 5 out of 5 by from It worked very nice on WB with Drosophila tissues It worked very nice on WB with Drosophila tissues.
Date published: 2015-05-19
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