cyclin A 항체 (H-3) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 cyclin A and cyclin A1을 검출할것을 권장합니다.

cyclin A 항체 (H-3): sc-271645

cyclin A 항체 (H-3) is rated 4.2 out of 5 by 10.
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데이터 시트

    • cyclin A 항체(H-3) 는 마우스 monoclonal IgG1κ, 24간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 cyclin A의 1-432 아미노산을 항원으로 사용하였습니다.
    • 안티-cyclin A 항체 (H-3)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 cyclin A and cyclin A1 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin A 항체 (H-3)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-271645 X, 200 µg/0.1 ml)이 있습니다.
    • cyclin A (H-3): sc-271645무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin A Antibody (H-3) for WB applications. These reagents are now offered in bundles with cyclin A Antibody (H-3) (see ordering information below).
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Can the X form of this antibody be used for applications other than ChIP and EMSA, such as Western Blot or IF?

Asked by: AbPolly
Thank you for your question. Yes, the X form of this antibody can be used in applications such as Western Blot or IF, the dilution factor just needs to be adjusted as it is ten times as concentrated as the standard form.
Answered by: Technical Support 15
Date published: 2017-01-26
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Rated 5 out of 5 by from 免疫沈降で使用 Immunoprecipitationで使用。5ul/sampleで用いた。良好に免疫沈降することができた。
Date published: 2020-06-19
Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysates Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Presence of CcnA protein in this cell line was previously confirmed using discontinued product sc-596
Date published: 2019-05-22
Rated 1 out of 5 by from no signal with HUVEC I got this sample 6 months ago to replace sc-596 for cyclin A with WB. With 35ug of HUVEC samples, I got NO signal after 5 minute exposure (primary 1:350, secondary 1:2000).
Date published: 2018-05-02
Rated 5 out of 5 by from Good ab for WB works well for western blotting with samples from human cultured cells
Date published: 2017-11-21
Rated 5 out of 5 by from Sehr gute ergebnisse WB 1-300 dilution ! I ordered 1 month ago, this cyclin a seems the best for mouse samples, I was working with primary cells isolated from young and old mice.
Date published: 2017-11-15
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Nice bands Tested several samples with cyclin A Antibody (H-3) and observed strong and specific band
Date published: 2017-01-19
Rated 5 out of 5 by from Works well for IF in mouse (NIH3T3) Works well for IF in mouse (NIH3T3), publishable data. -SCBT Publication Review
Date published: 2015-03-05
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