CPTI-M 항체 (D-8) WB, IP, IF 와ELISA으로 human origin의 CPTI muscle을 검출할것을 권장합니다.

CPTI-M 항체 (D-8): sc-515709

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데이터 시트

    • CPTI-M 항체(D-8) 는 마우스 monoclonal IgG1(kappa light chain) 이며 200 µg/ml으로 제공합니다.
    • specific for an epitope mapping between amino acids 685-705 within a cytoplasmic domain of of human origin
    • WB, IP, IF 와ELISA으로 human origin의 CPTI muscle을 검출할것을 권장합니다.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CPTI-M Antibody (D-8) for WB applications. These reagents are now offered in bundles with CPTI-M Antibody (D-8) (see ordering information below).
loading

How much sample do you recommend loading on the gel for Western blot detection using CPTI-M (D-8): sc-515709?

Asked by: Trav11
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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