Chk2 항체 (A-12) WB, IP, IF, IHC(P), FCM와 ELISA으로 mouse, rat와 human origin의 Chk2을 검출할것을 권장합니다.

Chk2 항체 (A-12): sc-5278

Chk2 항체 (A-12) is rated 4.2 out of 5 by 13.
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데이터 시트

    • Chk2 항체(A-12) 는 마우스 monoclonal IgG2bκ, 60간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Chk2에서 내부에 위치한 1-300 아미노산을 항원으로 사용하였습니다.
    • 안티-Chk2 항체 (A-12)는 WB, IP, IF, IHC(P), FCM and ELISA으로 mouse, rat and human유래의 Chk2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Chk2 항체 (A-12)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Chk2 (A-12): sc-5278무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Chk2 Antibody (A-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with Chk2 Antibody (A-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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When using this mouse monoclonal primary, what secondary should be used?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-16
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Rated 1 out of 5 by from It doesn't work in WB. We used it on WB at 1:100 diluition in BSA on B lymphocyte and we couldn't detect any bands.
Date published: 2020-10-13
Rated 5 out of 5 by from Good for western blot Worked nicely for Western blot with human and mouse cell lysates.
Date published: 2020-09-07
Rated 1 out of 5 by from It did not work We used in WB (1:100) in Panc1 and in human infected B lymphocytes, but we did not see any band.
Date published: 2020-02-10
Rated 5 out of 5 by from good product Good antibody for WB with clear background and single band.
Date published: 2018-11-02
Rated 4 out of 5 by from 可用于WB和IHC 我们购买此产品,用于WB和IHC实验,所用的细胞系为A2780,使用结果表明,该产品良好,无非特异性。
Date published: 2018-05-23
Rated 4 out of 5 by from this antibody works ok I used this antibody at a dilution 1:300 for my T98G cell lines O/N in milk in it worked
Date published: 2017-10-25
Rated 5 out of 5 by from Great Antibody Chk2 (A-12) works great in HeLa for Western blot (WB) as a positive control. And good nuclear staining in human lung tumor for IHC(P).
Date published: 2016-12-22
Rated 5 out of 5 by from Strong band in WB in mouse transfected ES cells Strong band in WB in mouse transfected ES cells. -SCBT Publication Review
Date published: 2015-04-30
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