Cdk9 항체 (D-7) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Cdk9을 검출할것을 권장합니다.

Cdk9 항체 (D-7): sc-13130

Cdk9 항체 (D-7) is rated 4.4 out of 5 by 17.
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데이터 시트

    • 안티-Cdk9 항체 D-7 는 마우스 monoclonal IgG2b κ Cdk9 항체, 84간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Cdk9에서 내부에 위치한 204-372 아미노산을 항원으로 사용하였습니다.
    • 안티-Cdk9 항체 (D-7)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Cdk9 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Cdk9 항체 (D-7)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • WB, IHC(P) 와 ELISA에 사용가능한 biotin결합제품 이 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-13130 X, 200 µg/0.1 ml)이 있습니다.
    • Cdk9 (D-7): sc-13130무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk9 Antibody (D-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdk9 Antibody (D-7) (see ordering information below).
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How has the specificity of this antibody to Cdk9 been validated?

Asked by: AbPolly
Thank you for your question. This specificity of this antibody has been tested by in house QC, including IF data with siRNA knockdown of Cdk9.
Answered by: Technical Support 6
Date published: 2017-01-11

Does this antibody have a blocking peptide associated with it?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein, unfortunately it is not possible to offer a blocking peptide for it.
Answered by: Technical Support 6
Date published: 2017-01-11
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Rated 4 out of 5 by from Great for western and IP Used at 1:500 dilution to detect endogenous protein in nuclear extract from HEK293T. 5ug antibody per 500ug nuclear extract IPed CDK9; cyclin T1 is detected with sc-271348 in CDK9 IP lane.
Date published: 2018-08-02
Rated 5 out of 5 by from Recommended for western blotting! It detected endogenous CDK9 in lysates from HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween-20, overnight at 4C).
Date published: 2018-06-18
Rated 1 out of 5 by from Did not work for Chip-seq We received a sample amount of D-7 antibody which we tested in our standard chip-seq experiment pipeline. We did not have sufficient amount to test various buffers. However, the IP with D-7 antibody did not produce any peaks after sequencing.
Date published: 2018-04-10
Rated 5 out of 5 by from CDK9 antibody I bought this item last month,it works pretty well.
Date published: 2017-12-28
Rated 5 out of 5 by from Nice antibody Strong and specific band in Western blotting, publishable quality.
Date published: 2017-12-07
Rated 4 out of 5 by from Good Ab for WB It works good for western-blot in breast cancer cells.
Date published: 2017-08-09
Rated 5 out of 5 by from Good CDK9 antibody This antibody worked well for WB and IP of mouse VSMC proteins.
Date published: 2017-03-09
Rated 4 out of 5 by from Good for Western blot It works for Western blot, but not good for co-IP.
Date published: 2017-01-16
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