Cdk4 항체 (DCS-35) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Cdk4을 검출할것을 권장합니다.

Cdk4 항체 (DCS-35): sc-23896

Cdk4 항체 (DCS-35) is rated 4.1 out of 5 by 29.
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데이터 시트

    • 안티-Cdk4 항체 DCS-35 는 마우스 monoclonal IgG1 κ Cdk4 항체, 142간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against full length recombinant human Cdk4, with epitope mapping to amino acids 1-20
    • 안티-Cdk4 항체 (DCS-35)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Cdk4 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Cdk4 항체 (DCS-35)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Cdk4 (DCS-35): sc-23896무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk4 Antibody (DCS-35) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdk4 Antibody (DCS-35) (see ordering information below).
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Hi. I have mouse sample and would like to do WB, using this Cdk4 Antibody (sc-23896) as my primary antibody. Can you please suggest a suitable secondary antibody? Or do you have other recommendation?

Asked by: faza
Thank you for your question. We recommend m-IgGκ BP-HRP: sc-516102 as a secondary antibody for this product. We also offer secondary binding proteins with other conjugation options, you can find them under this link: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Support Europe
Date published: 2020-08-11

Can you please send me a free sample of this Ab to validate its IF staining in my cells? Thank you!

Asked by: Afifim
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-23896 PE), fluorescein (sc-23896 FITC), Alexa Fluor® 488 (sc-23896 AF488) or Alexa Fluor® 647 (sc-23896 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 1 out of 5 by from poor staining in immunohistochemical study CDK4 Antibody (DSC-35): immunoperoxidase staining of formalin fixed, paraffin-embedded human dedifferentiated liposarcoma tissue showing poor nuclear staining of tumor cells.
Date published: 2021-07-14
Rated 4 out of 5 by from suitable for WB This antibody is suitable for WB (1:1000) in human cell lines
Date published: 2019-10-03
Rated 1 out of 5 by from Very low signal I tested the antibody in a few cell lines that I know express CDK4 (detected in WB by other antibodies). The signal for CDK4 was barely detectable after a long exposure with sensitive ECL. The customer service at Santa Cruz was great since they offered this as a replacement for another antibody that didn't work either but I had no results with two different antibodies.
Date published: 2018-08-08
Rated 5 out of 5 by from Nice band in WB Cdk4 had strong band in WB in human prostate cancer cell line LNcap
Date published: 2018-07-09
Rated 4 out of 5 by from OK antibody for IB! This Ab is surely working on IB of mouse cells, but not so good as sc-56277, higher background with multiple unspecific bands.
Date published: 2017-11-28
Rated 5 out of 5 by from very stable Using 1:600, it worked wonderfully in the WB experiment and successfully measured the expression of Cdk4, it very specific and strong signals.
Date published: 2017-11-01
Rated 1 out of 5 by from It does not work well in WB This antibody does not work well in WB. We recommend sc-56277.
Date published: 2017-09-20
Rated 5 out of 5 by from perfect antibody I use it in WB . SK-N-SH cell lysis used。 10% gel,electrophoresis: 30 mA,90 min。 transfer membrane :300 ma,45min。5% milk block,1::100 antibody overnight。1X TTBS wash 3 times ,second antibody 1:2000 ,1 h , temperature。then 1X TTBS wash 3 times。 1 min can appearance band,which is a specific band。
Date published: 2017-09-20
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