Cdk4 항체 (DCS-31) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 Cdk4을 검출할것을 권장합니다.

Cdk4 항체 (DCS-31): sc-56277

Cdk4 항체 (DCS-31) is rated 4.9 out of 5 by 15.
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데이터 시트

    • Cdk4 항체(DCS-31) 는 마우스 monoclonal IgG2aκ, 33간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • full length Cdk4 of human 의하고 제작된다
    • 안티-Cdk4 항체 (DCS-31)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 Cdk4 를 감지하는 데에 추천한다.; other Cdk types와 교차반응이 없습니다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Cdk4 항체 (DCS-31)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Cdk4 (DCS-31): sc-56277무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk4 Antibody (DCS-31) for WB applications. These reagents are now offered in bundles with Cdk4 Antibody (DCS-31) (see ordering information below).
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I need it for IP experiment. What is the specific protocol for this antibody conjugated to agarose beads?

Asked by: Anonymous
Thank you for your question. You can find our recommended IP protocol by following this link: https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Support Europe
Date published: 2020-09-07

I work primarily with zebrafish samples. Will this antibody work for me?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 1
Date published: 2017-01-13

I don't have any more of my donkey anti-mouse secondary. What can I use instead?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 1
Date published: 2017-01-13
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Rated 4 out of 5 by from Anticorpo Cdk4 Utilizzato in saggi di Western Blot, ha funzionato discretamente.
Date published: 2020-11-06
Rated 5 out of 5 by from Great anitbody! Very specific! no background! used 1:1000 dilution
Date published: 2018-03-01
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 5 out of 5 by from Great antibody for IB! This is a great antibody for IB, quite clean signal with little background. Better than sc-23896.
Date published: 2017-11-28
Rated 5 out of 5 by from Good antibody! I like this antibody as it has strong band binding and no background.
Date published: 2017-10-13
Rated 5 out of 5 by from It works excellent in WB This antibody works excellent in WB. Great antibody.
Date published: 2017-09-20
Rated 5 out of 5 by from GOOD FOR IB, IP I tested this antibody to IB(1:100), and IP (2ug/ml lysated protein) in HepG2, PLC/PRF/5, Huh7 and BT-549 cells. It recognizes the endogenous CDK4 protein, and also works for IP.
Date published: 2017-07-10
Rated 5 out of 5 by from WB clear band ,claen background and accurate protein position
Date published: 2017-06-05
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