Cdc6 항체 (D-1) WB, IP, IF 와ELISA으로 human origin의 Cdc6을 검출할것을 권장합니다.

Cdc6 항체 (D-1): sc-13136

Cdc6 항체 (D-1) is rated 4.3 out of 5 by 9.
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데이터 시트

    • Cdc6 항체(D-1) 는 마우스 monoclonal IgG1κ, 10간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Cdc6에서 내부에 위치한 257-560 아미노산을 항원으로 사용하였습니다.
    • Cdc6 항체 (D-1)는 WB, IP, IF and ELISA으로 human유래의 Cdc6 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Cdc6 항체 (D-1)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Cdc6 (D-1): sc-13136무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdc6 Antibody (D-1) for WB applications. These reagents are now offered in bundles with Cdc6 Antibody (D-1) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with Cdc6 (D-1): sc-13136 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody for Western blot This antibody performs very well for Western blot for multiple human cell lines.
Date published: 2020-01-05
Rated 1 out of 5 by from CDC6 Ab The antibody didn´t give me any signal using HCT116 nuclear extracts performed by standard WB procedure that worked for another Santa Cruz antibodies (for example sc-9964 – also against CDC6).
Date published: 2018-07-23
Rated 5 out of 5 by from Excellent antibody! Published for ChIP. This monoclonal Cdc6 Antibody (D-1) has excellent in-house data for Western blot and IF, and it has been published for ChIP (see PMID: 26872786).
Date published: 2017-02-07
Rated 5 out of 5 by from good antibody good antibody, used in WB on human samples
Date published: 2016-07-25
Rated 4 out of 5 by from This antibody works well and can be used This antibody works well and can be used for IP and Western Blot with human HeLa, T98G, U2-OS, HEK 293T, H1299 cell lines. -SCBT Publication Review
Date published: 2015-04-29
Rated 5 out of 5 by from Works well in Western Blot application with Works well in Western Blot application with the human HEF cell line. -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by from Strong band at correct molecular weight observed Strong band at correct molecular weight observed by Western Blot with HeLa nuclear extract. -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data in HeLa and S3 cells. -SCBT Publication Review
Date published: 2015-01-09
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