Myc/c-Myc 항체 (C-8) WB, IP, IF와 ELISA으로 mouse, rat, human와 avian origin의 c-Myc p67 and c-Myc tagged fusion proteins을 검출할것을 권장합니다.

Myc/c-Myc 항체 (C-8): sc-41

Myc/c-Myc 항체 (C-8) is rated 3.8 out of 5 by 10.
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데이터 시트

    • Myc/c-Myc 항체(C-8) 는 마우스 monoclonal IgG2aκ, 40간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • full length c-Myc of human 의하고 제작된다
    • 안티-Myc/c-Myc 항체 (C-8)는 WB, IP, IF and ELISA으로 mouse, rat, human and avian유래의 c-Myc p67 and c-Myc tagged fusion proteins 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Myc/c-Myc 항체 (C-8)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-41 X, 200 µg/0.1 ml)이 있습니다.
    • Myc/c-Myc (C-8): sc-41무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Myc/c-Myc Antibody (C-8) for WB applications. These reagents are now offered in bundles with Myc/c-Myc Antibody (C-8) (see ordering information below).
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Could I use this to detect endogenous c-Myc in mouse cells by flow cytometry? If not, which antibody do you recommend?

Asked by: Mariaaaaaa
Yes you can. We offer this antibody conjugated to either phycoerythrin or FITC for IF, IHC(P) and Flow Cytometry.
Answered by: Dallas Tech Service
Date published: 2021-04-07

When should the TransCruz reagent sc-41 X be used instead of the standard form sc-41?

Asked by: cjMara
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from 検出できません。 HEK293T細胞に過剰発現したMycタグ付きのタンパク質を検出できませんでした。他社抗体では検出できます。
Date published: 2020-10-15
Rated 5 out of 5 by from Very good antibody I used this antibody to detect transfected mouse c-Myc in MEF cell line, and it works well at 1:1000 dilution for western blot and 1:100 for imunofluorescence. I think that for immunofluorescence greater dilutions might also work well.
Date published: 2019-10-04
Rated 5 out of 5 by from Gives clear bands Bands show up nice and clearly. Used a 1:1000 dilution for the WB
Date published: 2019-03-21
Rated 4 out of 5 by from GOOD It worked well in 1:3000 in our stable HEK293t cell lines
Date published: 2018-04-03
Rated 1 out of 5 by from knockin mouse embryo IP 2ug of Ab from sample failed to IP myc-tag protein. 9E10 and 9E11 worked fine
Date published: 2017-12-27
Rated 4 out of 5 by from Buena resolución En uso para WB a una concentración de 1:1000 obtenemos una banda única.
Date published: 2017-10-10
Rated 5 out of 5 by from WB detection We use it for WB detection.rat brain shows positive band at 67kda.The loading amount at 60ug. Strong signal for different dilutions from 1:500 to 1:1000.
Date published: 2017-09-16
Rated 5 out of 5 by from strong band c-Myc antibody detected in Western blot, 1:1000 been used, the band is unique
Date published: 2017-04-25
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