Myc/c-Myc 항체 (C-33) WB, IP, IF와 ELISA으로 mouse, rat, human와 avian origin의 c-Myc p67 and c-Myc tagged fusion proteins을 검출할것을 권장합니다.

Myc/c-Myc 항체 (C-33): sc-42

Myc/c-Myc 항체 (C-33) is rated 4.3 out of 5 by 18.
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데이터 시트

    • Myc/c-Myc 항체(C-33) 는 마우스 monoclonal IgG1κ, 328간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • full length c-Myc of human 의하고 제작된다
    • 안티-Myc/c-Myc 항체 (C-33)는 WB, IP, IF and ELISA으로 mouse, rat, human and avian유래의 c-Myc p67 and c-Myc tagged fusion proteins 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Myc/c-Myc 항체 (C-33)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-42 X, 200 µg/0.1 ml)이 있습니다.
    • Myc/c-Myc (C-33): sc-42무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Myc/c-Myc Antibody (C-33) for WB applications. These reagents are now offered in bundles with Myc/c-Myc Antibody (C-33) (see ordering information below).
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Is this antibody suitable for detection of mouse c-Myc? I am planning to overexpress mouse c-Myc in mouse embryonic fibroblast cell line.

Asked by: IO11
Thank you for your question. c-Myc Antibody (C-33): sc-42 is recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2019-09-02

Can this antibody detect endogenous c-Myc protein as well as fusion proteins tagged with c-Myc?

Asked by: AbPolly
Thank you for your question. Yes, our c-Myc sc-42 is reactive with both endogenous c-Myc in samples, and with c-Myc tagged proteins in multiple applications.
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 5 out of 5 by from 抗体 用了好几家抗体,Santa一如既往的好。这个抗体效果太棒了,WB条带特别漂亮,价格也比较合理,推荐大家使用和购买
Date published: 2020-11-28
Rated 1 out of 5 by from 検出できません。 HEK293T細胞に過剰発現したMycタグ付きのタンパク質を検出できませんでした。他社抗体では検出できます。
Date published: 2020-10-15
Rated 5 out of 5 by from Gives a clear band at around 55 kDa Good antibody. We use a 1:1000 dilution for the western blot.
Date published: 2019-03-21
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 1 out of 5 by from knockin mouse embryo IP 2ug of Ab from sample failed to IP myc-tag protein. 9E10 and 9E11 worked fine.
Date published: 2017-12-27
Rated 2 out of 5 by from non specific bands I see lots of non specific bands when I try to detect endogenous Myc in U251 cells(1:200) and do not know which is the right band.
Date published: 2017-11-21
Rated 5 out of 5 by from Works very well The antibody worked great for us under the following conditions: 5% milk, 1:500 dilution, HEK whole cell lysates with RIPA lysis buffer. It is recommended for the detection of endogenous c-Myc
Date published: 2017-10-05
Rated 5 out of 5 by from Nice Western data Works better than my 9E10 antibody! very little background.
Date published: 2017-10-03
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