Benzonase® Nuclease 생물학적 활성을 갖는 단백질: 핵산의 소화를 핵산의 완상하는 것에 바람직한 응용에 이상적인 endonuclease

Benzonase® Nuclease (CAS 9025-65-4)

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Benzonase® Nuclease is rated 5.0 out of 5 by 2.
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Alternate Names: BenzNuclease
Application Benzonase® Nuclease supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol is an endonuclease ideal for applica
CAS 등록번호: 9025-65-4
순도: ≥90%
연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Benzonase® Nuclease is a solubilized version of Benzonase® Nuclease Lyophilized Powder (sc-391121). The Benzonase® enzyme is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.

Unit definition:
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.

Benzonase is a registered trademark of Merck KGaA.


References

1. Miller, M.D., et al. 1994. Nat. Struct. Biol. 1: 461-468. PMID: 7664065

2. Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-3287. PMID: 8078761

3. Ball, T.K., et al. 1992. Nucleic Acids Res. 20: 4971-4974. PMID: 1329033

4. Ball, T.K., et al. 1987. Gene. 57: 183-192. PMID: 3319779

5. Janning, P., et al. 1994. Rapid Commun. Mass Spectrom. 8: 1035-1040. PMID: 7696699

6. Molloy, M.P., et al. 1998. Electrophoresis. 19: 837-844. PMID: 9629924

7. Herbert, B.R., et al. 1998. Electrophoresis. 19: 845-851. PMID: 9629925

사용법 :
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of Benzonase® Nuclease is 1.3×106 ~ 1.5×106 unit/mg protein.
Formulation :
≥250 units/µL supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol.
Physical State :
Liquid
에서 유래된 :
Recombinant protein expressed in Escherichia coli
저장 :
Store at -20° C
연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.
WGK Germany :
1
MDL Number :
MFCD00131010
EC Number :
231-791-2

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PMID: # 33930289  Mendel, M.|Delaney, K.|Pandey, RR.|Chen, KM.|Wenda, JM.|Vågbø, CB.|Steiner, FA.|Homolka, D.|Pillai, RS.| et al. 2021. Cell.

PMID: # 32611815  Dong, C.|West, KL.|Tan, XY.|Li, J.|Ishibashi, T.|Yu, CH.|Sy, SMH.|Leung, JWC.|Huen, MSY.| et al. 2020. Proc Natl Acad Sci U S A. 117: 17019-17030.

PMID: # 31263572  Keskitalo, S.|Haapaniemi, EM.|Glumoff, V.|Liu, X.|Lehtinen, V.|Fogarty, C.|Rajala, H.|Chiang, SC.|Mustjoki, S.|Kovanen, P.|Lohi, J.|Bryceson, YT.|Seppänen, M.|Kere, J.|Heiskanen, K.|Varjosalo, M.| et al. 2019. NPJ Genom Med. 4: 14.

PMID: # 30692695  Charlesworth, CT.|Deshpande, PS.|Dever, DP.|Camarena, J.|Lemgart, VT.|Cromer, MK.|Vakulskas, CA.|Collingwood, MA.|Zhang, L.|Bode, NM.|Behlke, MA.|Dejene, B.|Cieniewicz, B.|Romano, R.|Lesch, BJ.|Gomez-Ospina, N.|Mantri, S.|Pavel-Dinu, M.|Weinberg, KI.|Porteus, MH.| et al. 2019. Nat. Med. 25: 249-254.

PMID: # 31135337  Bigot, N.|Day, M.|Baldock, RA.|Watts, FZ.|Oliver, AW.|Pearl, LH.| et al. 2019. Elife. 8:

PMID: # 31163614  Villafañez, F.|Gottifredi, V.|Soria, G.| et al. 2019. High Throughput. 8:

PMID: # 29565900  van Rooden, EJ.|Florea, BI.|Deng, H.|Baggelaar, MP.|van Esbroeck, ACM.|Zhou, J.|Overkleeft, HS.|van der Stelt, M.| et al. 2018. Nat Protoc. 13: 752-767.

PMID: # 30205044  Archer, TC.|Ehrenberger, T.|Mundt, F.|Gold, MP.|Krug, K.|Mah, CK.|Mahoney, EL.|Daniel, CJ.|LeNail, A.|Ramamoorthy, D.|Mertins, P.|Mani, DR.|Zhang, H.|Gillette, MA.|Clauser, K.|Noble, M.|Tang, LC.|Pierre-François, J.|Silterra, J.|Jensen, J.|Tamayo, P.|Korshunov, A.|Pfister, SM.|Kool, M.|Northcott, PA.|Sears, RC.|Lipton, JO.|Carr, SA.|Mesirov, JP.|Pomeroy, SL.|Fraenkel, E.| et al. 2018. Cancer Cell. 34: 396-410.e8.

PMID: # 29303417  Farrokhi, A.|Pakyari, M.|Nabai, L.|Pourghadiri, A.|Hartwell, R.|Jalili, R.|Ghahary, A.| et al. 2018. Tissue Eng Part A. 24: 955-967.

PMID: # 30154153  Högström, J.|Heino, S.|Kallio, P.|Lähde, M.|Leppänen, VM.|Balboa, D.|Wiener, Z.|Alitalo, K.| et al. 2018. Cancer Res. 78: 5820-5832.

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