ANO1 항체 (C-5) WB, IP, IF, IHC(P) 와ELISA으로 human origin의 ANO1을 검출할것을 권장합니다.

ANO1 항체 (C-5): sc-377115

ANO1 항체 (C-5) is rated 3.8 out of 5 by 9.
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데이터 시트

    • 안티-ANO1 항체 C-5 는 마우스 monoclonal IgG2b κ ANO1 항체, 7간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 ANO1에서 에 가까운 182-222 아미노산을 항원으로 사용하였습니다.
    • ANO1 항체 (C-5)는 WB, IP, IF, IHC(P) and ELISA으로 human유래의 ANO1 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-ANO1 항체 (C-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ANO1 (C-5): sc-377115무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for ANO1 Antibody (C-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with ANO1 Antibody (C-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I am using ANO1 (C-5): sc-377115 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from ANO1 antibody (C5) from Santa Cruz Biotechnology These antibodies show a band of a wrong molecular weight on Western blotting. Human ANO1 cannot be below 90 kDa - it should be around 140 kDa.
Date published: 2019-04-03
Rated 1 out of 5 by from ANO1 Antibody (C-5) Wrong molecular weight of ANO1 on Westerns.It cannot be 90K.
Date published: 2019-04-03
Rated 5 out of 5 by from Very good for immunofluorescence I tested this antibody in immunofluorescence using 2% PFA-fixed and permeabilized (0.3% TritonX-100) cells that express high endogenous levels of ANO1 and it worked very well at 1:100.
Date published: 2017-09-26
Rated 4 out of 5 by from Good staining in Western Blot Staining of human kidney cells showed a very strong and unique band at the expected size.
Date published: 2017-07-03
Rated 3 out of 5 by from Hasn't worked...yet.... I purchased this antibody for western blotting. So far it hasn't worked, but I hope to optimize the conditions and get it working...
Date published: 2017-02-24
Rated 5 out of 5 by from Good choice for IHC Specific membrane and cytoplasmic staining of glandular cells seen in IHC with paraffin-embedded tissue.
Date published: 2017-01-23
Rated 5 out of 5 by from It works well on human vascular smooth muscle It works well on human vascular smooth muscle cells by WB, IF, IP.
Date published: 2015-09-30
Rated 5 out of 5 by from Excellent immunoperoxidase membrane and cytoplasmic Excellent immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-02-21
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