TCR beta抗体(G-11) human由来のTCR β WB, IP, IF, IHC(P) と ELISAでの検出にはお勧めします

TCR β抗体(G-11): sc-5277

TCR β抗体(G-11)は、 10件のレビューによって、 5つ星のうち 4.9と 評価されています。
  • y_2021, m_8, d_4, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_ja_JP, sid_5277, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 116ms
  • REVIEWS, PRODUCT
loading
5
9
4
1
3
0
2
0
1
0

データシート

    • TCR β抗体 (G-11)はマウスモノクローナルIgG1 κです。200 µg/mlで提供
    • raised against the constant region of TCR β of human origin
    • TCR beta抗体 (G-11) human由来のTCR β WB, IP, IF, IHC(P) と ELISAでの検出にはお勧めします
    • 反-TCR beta抗体 (G-11) IP用のアガロース、に共役での利用可能です。WB、IHC(P)とELISA用のHRP、IF、IHC(P)とFCM用のphycoerythrin または FITC、に共役での利用可能です。
    • WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor® 488Alexa Fluor® 546Alexa Fluor® 594 または Alexa Fluor® 647、に共役での利用可能です。
    • WB (NIR)、IF、FCMとLI-COR®/Odyssey®、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムで近赤外(NIR)検出法用のAlexa Fluor® 680 または Alexa Fluor® 790、に共役での利用可能です。
    • TCR β (G-11): sc-5277無料の10 µgサンプルを受け取るには、ご連絡ください。
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP は推奨セカンダリー検出試薬 TCR β 抗体 (G-11) for WB and IHC(P) applications. これらの試薬と TCR β 抗体 (G-11) (下記の注文情報をご覧ください)とセットになっています。
loading

Does it recognize mouse TCR beta or only human

質問者: Maria Teresa
Thank you for your question. This antibody has not been tested for mouse and rat use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
回答者: Tech Service
投稿日: 2019-03-01

I tried this Ab for IF with dilution 1:50 on 4% PFA fixed Jurkat cells but I didn't see the specific staining. How should I troubleshoot it?

質問者: KanyaratP
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
回答者: Technical Support
投稿日: 2019-01-14

Any idea how much to use TCR β Antibody (G-11) for Flow Cytometry? I tested at final staining concentration 5ug/ml and failed to see any positive stain in purified human T-cells.

質問者: JonathanH
Thank you for your question. Below is a link to our recommended protocol for flow cytometry. Please contact our Technical Service Department if you would like help troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
回答者: Technical Support
投稿日: 2017-10-12

Does TCR β (G-11): sc-5277 react with canine samples?

質問者: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
回答者: Technical Support 15
投稿日: 2017-01-16

Why is TCR β (G-11): sc-5277 the recommended alternative to the now discontinued TCR β (H-197): sc-9101?

質問者: Trav11
Thank you for your question. The monoclonal primary antibody TCR β (G-11): sc-5277 offers a higher degree of specificity and essentially eliminates all lot-to-lot variation. It is also warrantied for all of the same applications as the discontinued polyclonal antibody: WB, IP, IF, and IHC(P). If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
回答者: Technical Service 11
投稿日: 2017-01-16
  • y_2021, m_8, d_4, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_ja_JP, sid_5277, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 113ms
  • QUESTIONS, PRODUCT
によって、 5つ星のうち 5 と評価されています。 还不错 虽然订购过程很郁闷,但是真正发货还是很快到货的。使用的时候基本上按照说明书的稀释比使用,基本能满足要求
投稿日: 2021-04-06
によって、 5つ星のうち 5 と評価されています。 IHC I do IHC with this antibody on human brain sections. I got excellent specific staining of interneurons without any background. The antibody dilution: 1:200.
投稿日: 2017-08-19
によって、 5つ星のうち 5 と評価されています。 The sc-5277 TCR beta antibody has good specificity, it detect this sample from dilution 1:500, clean background.
投稿日: 2017-05-07
によって、 5つ星のうち 5 と評価されています。 IHC-P This antibody could be used to do IHC-P in human samples.
投稿日: 2017-02-10
によって、 5つ星のうち 5 と評価されています。 Outstanding product !! Better than polyclonal alternative for WB, IF and IHC applications
投稿日: 2017-01-20
によって、 5つ星のうち 5 と評価されています。 Good for IHC and IF This TCR beta antibody shows good staining for IF and IHC in human samples.
投稿日: 2017-01-12
によって、 5つ星のうち 5 と評価されています。 Robust bands in western blot analysis of Robust bands in western blot analysis of TCR expression in MOLT-4 and CCRF-CEM whole cell lysates. -SCBT QC
投稿日: 2015-06-30
によって、 5つ星のうち 5 と評価されています。 Great IF images using this antibody with methanol Great IF images using this antibody with methanol-fixed CCRF-CEM cells -SCBT QC
投稿日: 2015-06-16
  • y_2021, m_8, d_4, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_ja_JP, sid_5277, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT